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Biocolor 可溶性胶原检测试剂盒

Biocolor 可溶性胶原检测试剂盒
Biocolor Sircol Soluble Collagen Assay

    采用与胶原特异性结合的Sircol染料,主要用于定量检测哺乳动物组织中抽提的酸溶性胶原和体外培养哺乳动物细胞释放到培养基中的胶原。
    溶于酸、胃蛋白等的可溶性胶原均可检测,具体可检测的胶原类型为I-V型,VI-XIV型胶原也可检测,但由于难以制备标准曲线,还无法定量。Sircol染料不能区分不同类型的胶原,但可与胶原的[Gly-X-Y]n螺旋结构特异性地结合。随着胶原变性为凝胶,该染料与胶原的结合力减弱。非脊椎动物与胶原由于变性温度较低、羟脯氨酸残基较少等原因,结合染料的能力较弱,需要制备种属特异性的纯化标准品,以绘制标准曲线。
 
实验步骤
绘制标准曲线
待测样本的制备:取50ul样本容易调整体积到100ul进行反应。根据反应的结果对样本浓度进行调整,使其在标准曲线范围内;
胶原—染料复合物的获得:加入1ml Sircol染料,混匀离心后,既得胶原—染料复合物,弃上清,去掉未结合的染料
加入1ml 染料释放剂,将胶原—染料复合物中的染料释放出来
吸光度测定:用分光光度计540nm出定量测定。或使用colorimeter测定

产品订购
     Product Code: S1000    120 assays
     Product Code: S5000    475 assays

货 号 品 名 规 格 单价(元) 厂 家
A1000 APOPercentage Assay,6×96 well format   ¥5,269 Biocolor
B1000 Blyscan sGAG Assay,(120 assays),[standard kit Size]   ¥6,105 Biocolor
B1005 Blyscan Dye Reagent,(3x120ml)   ¥6,364 Biocolor
B1010 Blyscan sGAG Reference Standard,(100ug/ml;3x5ml)   ¥3,849 Biocolor
B1015 Blyscan sGAG Isolation & Concentration Pack,(100samples)   ¥4,418 Biocolor
B1500 Blyscan Dye & Dissociation Reagent,[2x2x120ml]   ¥6,364 Biocolor
B3000 Blyscan sGAG Assay,(475 assays),[Large Economy Kit]   ¥13,613 Biocolor
F2000 Fastin Elastin Assay,(120 assays),[Standard Kit Size]   ¥6,542 Biocolor
F2010 Fastin Elastin Reference Standard,(1mg/ml;3x5ml)   ¥4,009 Biocolor
F2300 Fastin Dye Reagent,(3x120ml)   ¥10,852 Biocolor
F4000 Fastin Elastin Assay,(475 assays),.[Large Economy Kit]   ¥14,800 Biocolor
S1000 Sircol Collagen Assay,(120 assays) [standard kit Size]   ¥6,007 Biocolor
S1005 Sircol Dye Reagent,(3x120ml)   ¥6,774 Biocolor
S1007 Sircol Dye Realease Reagent,(3x150ml)   ¥4,427 Biocolor
S1010 Sircol Bovine Collagen Reference Standard,(0.5mg/ml,3x5ml)   ¥4,427 Biocolor
S1011 Sircol Rat Collagen Reference Standard,(0.5mg/ml,3x5ml)   ¥4,427 Biocolor
S1111 Sircol Collagen Assay,(120 assays) [Rat Collagen]   ¥6,007 Biocolor
S1500 Sircol Dye& Dye Release Reagent,[2x2x120ml]   ¥7,050 Biocolor
S5000 Sircol Collagen Assay,(475 assays),[large Economy Kit]   ¥13,195 Biocolor