产品英文名称：Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit
产品中文名称：Stemcell 19758 EasySep™ 小鼠上皮细胞富集试剂盒

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 产品简介
Stemcell 19762 EasySep™ Mouse Neutrophil Enrichment Kit 旨在通过阴性选择从新鲜解离的小鼠乳腺组织中分离上皮细胞。不需要的细胞有针对性的被非上皮细胞(CD4, CD5, CD11c, CD45R/B220, CD49b, CD117, TER119, F4/80)针对的生物素抗体瞄准去除，。标记的细胞然后被抗生物素和葡聚糖的四聚体抗体配合物识别。这些细胞被绑定到磁性粒子，使用没有柱的EasySep™磁铁 分离。期望得到的细胞倒入到一个新的管。

Stemcell 19758 EasySep™ 小鼠上皮细胞富集试剂盒产品组分
Stemcell 19758 EasySep™ 小鼠上皮细胞富集试剂盒有三个部分
• EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 推荐使用
Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit is designed for the isolation of mouse epithelial cells from cell suspensions by negative selection

Stemcell 19758 EasySep™ 小鼠上皮细胞富集试剂盒所需设备
• EasySep™ Magnet (Catalog #18000), or
• "The Big Easy" EasySep™ Magnet (Catalog #18001)

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 保存方法
EASYSEP™ MOUSE EPITHELIAL CELL ENRICHMENT COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ BIOTIN SELECTION COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 实验资料
Start: 10.37 ± 0.50% of cells expressing one or more of the following markers: CD45, Ter119, CD31, and/or BP-1. Enriched: 1.12 ± 0.22% of cells expressing one or more of the following markers: CD45, Ter119, CD31, and/or BP-1.

The percentage of hematopoietic, endothelial, and fibroblast cells after separation is 1.12 ± 0.22%. Purity has been assessed by staining with goat anti-rat IgG-FITC, which recognizes the antibodies used to deplete cells expressing CD45, Ter119, CD31, and/or BP-1.

Typical EasySep™ Procedure for Mouse Cells


Stemcell 19758 EasySep™ 小鼠上皮细胞富集试剂盒相关产品订购信息
Stemcell  07912  Collagenase/Hyaluronidase  10X Collagenase/Hyaluronidase in DMEM 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  05610  EpiCult™-B Mouse Medium Kit  Medium for Mouse Mammary Epithelial Cell Culture 
Stemcell  05640  ProstaCult™ Mouse Medium Kit  Medium for the Culture of Mouse Prostate Epithelial Cells     
For research use only. Not intended for human or animal diagnostic or therapeutic uses.