Frizzled-8 Fc Fusion, human
规格:
数量:
价格:
折后价:
金牌价格:
联系客服:
产品英文名称:Frizzled-8 Fc Fusion, human
产品中文名称:人Frizzled-8 Fc Fusion蛋白
Frizzled-8 Fc Fusion, human产品介绍:
Frizzled-8是WNT蛋白10个哺乳类卷曲受体之一。卷曲蛋白细胞外的半胱氨酸富集区与WNTs的密切关系可达到nM级别。当被作为重组蛋白时,Frizzled-8的细胞外区域就成为可溶的受体,以阻止细胞中WNTs与Frizzled的结合。
StemRD公司的Frizzled-8细胞外主蛋白是与人IgG1细胞Fc片段的N-终端相融合的。这种融合保证了体内外蛋白的稳定性,以及重组蛋白二聚体对WNTs的高亲和性。研究基因科技的科学家曾使用与之类似的蛋白Frizzled-8 Fc融合,证实了动物模型中WNT封锁物的抗肿瘤效果(DeAlmeida VI et al,Cancer Res.2007,67:5371)。
用无血清无蛋白的培养基培养CHO细胞,看其基因表达情况。使用蛋白A色谱分析法对特定培养基条件下的融合蛋白进行纯化。
Frizzled-8 Fc Fusion, human产品英文简述:
Frizzled-8 is one of the 10 mammalian Frizzled receptors for WNT proteins. The extracellular cycteine rich domain of Frizzleds bind to WNTs with nM affinity. When supplied as recombinant protein, this extracellular domain of Frizzled-8 acts as a soluble receptor, blocking the binding of WNTs to Frizzled on the cells.
StemRD's Frizzled-8 extracellular domain protein is fused to the Nterminus of the Fc domain of human IgG1. This fusion allows stabilization of the protein in vitro and in vivo and dimerization of the recombinant protein rendering high-affinity binding to WNTs. The Frizzled-8 Fc fusion has been used by Genentech scientists to show anti-tumor effect of WNT blockade in animal models (DeAlmeida VI et al, Cancer Res. 2007, 67:5371).
Expression is from CHO cells cultured in serum-free and protein-free medium. Purification of the fusion protein from conditioned culture medium is performed using protein A chromatography.
• Purity:Greater than 95% as determined by SDS-PAGE analysis
• Biological Activity:The activity was determined by using a TCF reporter gene assay in cultured
human cells. The IC50 ranges from 100 - 200 ng/ml in the inhibition of 100 ng/mL WNT-3a activity.
OriGen CP-70 CryoPur-DMSO 细胞冻存液 二甲基亚砜 进口USP级 CE认证 CP70
¥1Lifeline LL-0002 VascuLife EnGS Complete Kit 人内皮细胞培养基套装
¥1Anti-Human CD4 PerCP-Cyanine5.5 人CD4抗体
¥4,188Corning 88-571-kit Natural Killer Cell Serum-free Culture Kit II NK细胞活化扩增培养基套装
¥0Cellartis Y50200 MSC Xeno-Free Culture Medium 人间充质干细胞培养基,无外源无需包被
¥1Origen CS250 Freezing Bags vol-max 30-70mL 冻存袋 细胞治疗用药包材
¥1【爆款推荐】适合再生医疗研究用的人间充质干细胞培养基
【专利热卖产品】Takara Cellartis iPS定向分化肝脏细胞专利技术解析
人间充质干细胞培养基怎么选,看这三个点就可以了!
人胚胎干细胞(ESC)/诱导多能干细胞(iPSC)培养推荐:Cellartis® DEF-CS™系列产品 授权代理
【CRISPR/Cas9系统】人iPS细胞推荐基因编辑工具
为什么说PAXgene是RNA保存必选产品,数据来说话!
RNA转录组学研究对临床医学的重大意义【附带RNA样本保存神器】
PLTGOLD500GMP人血小板裂解物,为细胞培养和药物生产提供最佳环境
要养支气管上皮细胞?Lifeline上皮细胞培养基助您一臂之力
分析RNA降解的常见原因,如何防止RNA降解