Gibco® PSC Neural Induction Medium (prototype)
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英文名称:Gibco® PSC Neural Induction Medium (prototype)
中文名称:Gibco® PSC神经诱导培养基(prototype)
Gibco® PSC神经诱导培养基 产品介绍:
Gibco® PSC神经诱导培养基是一种无血清培养基,可高效诱导人多能干细胞(PSCs)(7天)分化为神经干细胞(NSCs)。适用培养的细胞源包括诱导多能干细胞、多能干细胞、胚胎干细胞和神经细胞等。与现有的方法不同,使用Gibco® PSC神经诱导培养基时不需要经过形成胚状体(EB)中间环节,因此可节约时间和精力,不会引发变异。使用Gibco® PSC神经诱导培养基培养成的神经干细胞(NSCs)具有高效表达NSC标志物 (Sox1, Sox2, and Nestin),同时可进一步分化成其它神经细胞类型。”prototype” 形式的培养基产品意味着这些产品通常都依照cGMP进行过实时定量研究。
产品组份:
• Neurobasal® Medium 490 mL
• Gibco® Neural Induction Supplement 10 mL
产品特性:
• 快速诱导NSCs:7天内完成高效神经诱导,无需EB
• 大量生成NSCs:100万PSCs可生成2000万NSCs
• 高质量的NSCs:收获的NSCs可冷冻保藏、扩增和分化
Gibco® PSC Neural Induction Medium (prototype) :
Rapid Induction of NSCs: Gibco® PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, Gibco® PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.
Scalable Production of NSCs: Gibco® PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune®-iPS Sendai Reprogramming Kit.
Generation of High Quality NSCs: Neural stem cells generated using Gibco® PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes
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