L.lactis HCP ELISA kit
产品英文名称：L.lactis HCP ELISA kit
产品中文名称：L.lactis HCP ELISA ELISA 试剂盒
L.lactis HCP ELISA kit 的应用
L.lactis HCP ELISA 试剂盒的英文描述
This kit has been specifically developed and validated for use in detecting L.lactis HCPs as contaminants in products produced by recombinant expression in Lactococcus lactis. The antibodies used in this kit were generated against and affinity purified from HCPs obtained from the spent culture media of a null cell line. Our assay validation has determined that the LOD for this kit is ~0.125ng/mL.
L.lactis HCP ELISA kit 的使用步骤规则
The L. lactis assay is a two-site immunoenzymetric assay.Samples containing L. lactis HCPs are reacted with a horseradish peroxidase (HRP) enzyme labeled anti-L. lactis antibody simultaneously in microtiter strips coated with an affinity purified capture anti-L. lactis antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody.The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of L. lactis HCPs present.
L.lactis HCP ELISA 试剂盒的保存和稳定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
* Reconstituted wash solution is stable until the expiration date of the kit.
L.lactis HCP ELISA kit 的使用注意事项
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