产品英文名称：SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric*
产品中文名称：SensoLyte®620 HCV 蛋白酶荧光检测试剂盒

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 产品介绍
丙型肝炎病毒（HCV）NS3／4A蛋白酶是病毒在NS3-NS4A，ns4a-ns4b，ns4b-ns5a，和ns5a-ns5b这些位点非结构蛋白裂解所需的。这些裂解的病毒蛋白的成熟至关重要。因此，该蛋白酶成为开发抗HCV药物的关键目标之一。该SensoLyte® 620 HCV蛋白酶检测试剂盒提供了高流率的一个方便的测定。HCV NS3/4A蛋白酶抑制剂和HCV NS3/4A连续定量筛选使用Hilyte氟蛋白酶活性TR/QXL™610荧光共振能量转移（FRET）肽。这个肽序列是来自裂解位点NS4A / NS4B。在苦恼的肽，HiLyte Fluor™r荧光淬灭通过QXL™610来完成。在分裂成两个独立的片段的HCV NS3/4A蛋白酶（方案1），HiLyte Fluor™TR是可以回收的，可监测激发/发射= 591 nm和622 nm。与附近的红色波长，Hilyte  Fluor™TR信号避免大多数测试化合物的干扰，因此是理想的流率HCV NS3/4A蛋白酶抑制剂的筛选。

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 的组分
Component A    HCV NS3/4A protease substrate                                      120 μL
                           Ex/Em=591 nm/622 nm upon cleavage
Component B    HiLyte Fluor™TR, fluorescence reference standard      100 μM, 5 μL
                           Ex/Em=591 nm/622 nm
Component C    2X Assay buffer                                                                  10 mL
Component D    Stop solution                                                                      10 mL
Component E    DTT                                                                                    1 M, 0.5 mL
Component F    Pep4AK                                                                               50 μL, 600 μM
                           HCV NS3 protease cofactor                         

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 的保存
• Store all kit components at -20°C
• Protect Components A and B from light
• Components C and D can be stored at room temperature for convenience

SensoLyte® 620 HCV 蛋白酶荧光检测试剂盒的英文描述
The SensoLyte® 620 HCV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte Fluor™ TR (fluorophore) and QXL™ 610 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of Hilyte Fluor™ TR is quenched by QXL™ 610. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of Hilyte Fluor™ TR is recovered and can be continuously monitored at excitation/emission = 590 nm/620 nm. With near red emission wavelength, this HiLyte Fluor™ TR/QXL™ 610-based FRET peptide avoids the autofluorescence interference from most test compounds. This feature makes this FRET peptide an ideal substrate for screening HCV protease inhibitors since some of screening compounds have strong autofluorescence at shorter wavelength, making other FRET peptide substrates inappropriate. The assays are performed in a convenient 96-well or 384-well microplate format.
The kit contains:
• HiLyte Fluor™ TR/QXL™ 610-based FRET peptide substrate (Ex/Em=590/620 nm upon cleavage)
• Assay buffer
• Fluorescence reference standard for calibration
• A detailed protocol
Kit Size: 100 assays

SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric* 系列产品订购信息
AnaSpec  71146  SensoLyte® 620 HCV Protease Assay Kit *Fluorimetric*  1 kit         
AnaSpec  72169-5  HCV NS3/4A protease genotype 4d, recombinant  5 µg            
AnaSpec  72168-5  HCV NS3/4A protease genotype 3a, recombinant  5 µg             
AnaSpec  72166-5  HCV NS3/4A protease genotype 1a, recombinant  5 µg             
AnaSpec  61017-10  HCV NS3/4A protease genotype 1b, recombinant  10 µg              
AnaSpec  72168-10  HCV NS3/4A protease genotype 3a, recombinant  10 µg             
AnaSpec  61017-5  HCV NS3/4A protease genotype 1b, recombinant  5 µg            
AnaSpec  72166-10  HCV NS3/4A protease genotype 1a, recombinant  10 µg              
AnaSpec  72169-10  HCV NS3/4A protease genotype 4d, recombinant  10 µg              
AnaSpec  72167-10  HCV NS3/4A protease genotype 2a, recombinant  10 µg              
AnaSpec  72167-5  HCV NS3/4A protease genotype 2a, recombinant  5 µg