Mouse IFN-γ ELISPOT Set
产品英文名称：Mouse IFN-γ ELISPOT Set
产品中文名称：大鼠干扰素-γ ELISPOT set
Mouse IFN-γ ELISPOT Set 的成分
1.Capture Antibody: Anti-mouse IFN-γ
2.Detection Antibody: Biotinylated anti-mouse IFN-γ
3.Enzyme Reagent: Streptavidin-horseradish peroxidase conjugate (SAv-HRP)
4.Standard: Recombinant mouse IFN-γ (lyophilized)
Mouse IFN-γ ELISPOT Set 推荐使用步骤
1. Coat microwells with 100 μL per well of Capture Antibody diluted in Coating Buffer. For recommended antibody coating dilution, see
lot-specific Instruction/Analysis Certificate. Seal plate and incubate overnight at 4° C.
2. Aspirate wells and wash 5 times with ≥ 300 μL/well Wash Buffer. After last wash, invert plate and blot on absorbent paper to remove any residual buffer.
3. Block plates with ≥ 200 μL/well Assay Diluent. Incubate at RT for 1 hour.
4. Aspirate/wash as in step 2.
5. Prepare standard and sample dilutions in Assay Diluent. See “Standards Preparation and Handling”.
6. Pipette 100 μL of each standard, sample, and control into appropriate wells.Seal plate and incubate for 2 hours at RT.
7. Aspirate/ wash as in step 2, but with 5 total washes.
8. Add 100 μL of Working Detector (Detection Antibody + SAv-HRP reagent) to
each well. Seal plate and incubate for 1 hour at RT.
9. Aspirate/ wash as in step 2, but with 10 total washes. NOTE: In this final wash step, soak wells in wash buffer for 30 seconds to 1 minute for each wash.
10. Add 100 μL of Substrate Solution to each well. Incubate plate (without plate sealer) for 30 minutes at room temperature in the dark.
11. Add 50 μL of Stop Solution to each well.
12. Read absorbance at 450 nm within 30 minutes of stopping reaction. If wavelength correction is available, subtract absorbance at 570 nm from absorbance 450 nm.
小鼠干扰素-γ ELISPOT set 保存方法
1. Store unopened reagents at 2-8°C. Do not use reagents after expiration date, or if turbidity is evident.
2. Before use, bring all reagents to room temperature (18-25°C).Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until expiration date. See below for reconstituted standard storage information.
小鼠干扰素-γ ELISPOT set 的优越性
•适用于冷冻/解冻的生物样品 •与其他检验方法兼容。例如，细胞分析。BD™ ELISPOT可以转移克隆，增殖实验，流式细胞术或其他分析方法。
小鼠干扰素-γ ELISPOT set 使用注意事项
1. Reagents which contain preservatives may be toxic if ingested, inhaled, or in contact with skin.
2. Handle all serum and plasma specimens in accordance with NCCLS guidelines for preventing transmission of blood-borne infections.
3. Capture Antibody contains < 0.1% sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Detection Antibody contains BSA and ProClin.-150 as a preservative.
5. Enzyme Reagent contains BSA and ProClin.-150 as a preservative.
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