Human IFN-γ ELISPOT Pair
产品英文名称：Human IFN-γ ELISPOT Pair
产品中文名称：人干扰素-γ Elispot Pair
Human IFN-γ ELISPOT Pair 产品介绍
The enzyme-linked immunospot (ELISPOT) assay is a powerful tool for detecting and enumerating individual cells that secrete a particular protein in vitro. Based on the sandwich ELISA, the ELISPOT assay derives its specificity and sensitivity by employing high affinity capture and detection antibodies and enzyme-amplification. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete other effector molecules, such as cytokines. The sensitivity of the assay lends itself to measurement of even very low frequencies of cytokine producing cells (e.g., 1/300,000). Unique strengths of the assay include high sensitivity, high throughput, high content analysis, minimal volume of biological material required, applicability to frozen/thawed biological samples, and compatibility with other assays. For example, cells analyzed by ELISPOT can be transferred for cloning, proliferation assays, flow cytometry, or other methods of analysis.
This product contains sufficient reagents for five 96-well plates, including unlabelled anti-cytokine capture antibody (no azide/low endotoxin format); biotinylated anti-cytokine detection antibody and a Certificate of Analysis that provides lot-specific optimal reagent concentrations.
Human IFN-γ ELISPOT Pair 的保存方法
Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.
Human IFN-γ ELISPOT Pair 的推荐使用步骤
A. BD™ ELISPOT Streptavidin Horseradish Peroxidase (Cat. No. 557630) is recommended.
B. Coating Buffer (1X Phosphate Buffered Saline [PBS]): 8 g NaCl; 0.2 g KCl; 1.44 g Na2HPO4•7H2O, 0.24 g KH2PO4; dissolved in H2O to a final volume of 1 liter. Adjust pH to 7.2, sterile-filter (0.2 μm-sized pore filter) and store at 4oC.
C. Blocking Solution: complete tissue culture medium (e.g. RPMI 1640 containing 10% Fetal Bovine Serum [FBS] and 1% Penicillin-Streptomycin-L-Glutamine [Gibco-BRL No. 10378-016]).
D. Wash Buffer I: 1X PBS containing 0.05% Tween-20 (0.5 mL Tween-20 per 1 L PBS).
E. Wash Buffer II: 1X PBS
F. Dilution Buffer: 1X PBS containing 10% FBS.
G. BD™ ELISPOT AEC Substrate Set (Cat. No. 551951) .
Human IFN-γ ELISPOT Pair 的优越性
•适用于冷冻/解冻的生物样品 •与其他检验方法兼容。例如，细胞分析，BD™ ELISPOT可以转移克隆，增殖实验，流式细胞术或其他分析方法。
Human IFN-γ ELISPOT Pair 使用注意事项
1. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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