EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion
产品英文名称: EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion
产品中文名称: EasySep™ 没有CD16耗尽的人单核细胞富集试剂盒
The EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion is designed to isolate monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. The CD14+CD16+ subset of monocytes (~10% in blood of healthy individuals) has characteristics of tissue macrophages and expands greatly in acute and chronic inflammatory disease. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD19, CD20, CD56, CD66b, CD123, glycophorin A and dextran-coated magnetic particles. The cocktail also contains and antibody to human Fc receptor to minimize nonspecific binding. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which removal of all CD16+ cells is desired, we recommend the EasySep™ Human Monocyte Enrichment Kit (Catalog #19059), which contains anti-CD16.
EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Recommended for
The isolation of human monocytes and CD14+CD16+ monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection
• EasySep™ Human Monocyte Enrichment Cocktail without CD16 Depletion, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL
EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion 保存方法
EASYSEP™ HUMAN MONOCYTE ENRICHMENT COCKTAIL WITHOUT CD16
EASYSEP™ D MAGNETIC PARTICLES FOR MONOCYTES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
FACS Histogram Results Using EasySep® Human Monocyte Enrichment Kit Without CD16 Depletion
Starting with freshly prepared peripheral blood mononuclear cells, the CD14+ cell content of the enriched fraction typically ranges from 73% - 81%. Slightly lower CD14+ cell purities may be obtained from samples that contain a large number of CD16+ cells.
Typical EasySep™ Procedure for Human Cells
Stemcell 10406 CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated
Stemcell 07801 Lymphoprep™ Density Gradient Medium for the Isolation of Mononuclear Cells
Stemcell 18000 EasySep™ Magnet Immunomagnetic Column-Free Magnet
Stemcell 18001 The Big Easy EasySep™ Magnet Immunomagnetic Column-Free Magnet Stemcell 18002 Stemcell Easy 50 EasySep™ Magnet Immunomagnetic Column-Free Magnet Stemcell 19059 EasySep™ Human Monocyte Enrichment Kit Immunomagnetic Negative Selection Kit
Stemcell 20000 RoboSep™ RoboSep™ - The Fully Automated Cell Separator
Stemcell 60004 Anti-Human CD14 Antibody, Clone M5E2 Mouse Monoclonal Antibody Against Human, Rhesus, Cynomolgus CD14
Stemcell 60012 Anti-Human CD32 Antibody, Clone IV.3 Mouse Monoclonal Antibody Against Human CD32
Stemcell 60018 Anti-Human CD45 Antibody, Clone HI30 Mouse Monoclonal Antibody Against Human, Chimpanzee CD45
Stemcell 60026 Anti-Dextran Antibody, Clone DX1 Mouse Monoclonal Antibody Against Dextran Against Human, Rhesus, Cynomolgus CD14
For research use only. Not intended for human or animal diagnostic or therapeutic uses.
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