产品英文名称：Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit
产品中文名称：Stemcell 19782 EasySep™ 小鼠CD4+CD25+调节性T细胞分离试剂盒

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 产品简介
Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 通过阳性选择从小鼠脾细胞的单细胞悬液或其他组织中分离CD4+ CD25+ T细胞。 CD4+ T细胞富集使用EasySep™小鼠CD4+ T细胞预富集试剂盒的阴性选择。然后CD25+细胞从预富集的细胞中使用EasySep™小鼠CD25阳性选择试剂盒。富集所有的CD4+ T细胞时，不进行CD25+细胞的选择，我们建议的EasySep™小鼠CD4+ T细胞富集试剂盒（产品编号19752）。

Stemcell 19782 EasySep™ 小鼠CD4+CD25+调节性T细胞分离试剂盒产品组分
Stemcell 19782 EasySep™ 小鼠CD4+CD25+调节性T细胞分离试剂盒有两个部分
• EasySep™ Mouse CD4+ T Cell Pre-Enrichment Kit
• EasySep™ Mouse CD25 Positive Selection Kit

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 推荐使用
Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit is designed for the isolation of mouse CD4+CD25+ cells from single cell suspensions of splenocytes or other tissues by negative followed by positive selection

Stemcell 19782 EasySep™ 小鼠CD4+CD25+调节性T细胞分离试剂盒所需设备
• EasySep™ Magnet (Catalog #18000), or
• "The Big Easy" EasySep™ Magnet (Catalog #18001)

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 保存方法
EASYSEP® MOUSE CD4+ T CELL PRE-ENRICHMENT COCKTAIL
Product stable at 2 – 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 22°C), and should be refrigerated upon receipt.
EASYSEP® BIOTIN SELECTION COCKTAIL
Product stable at 2 – 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 22°C), and should be refrigerated upon receipt.
EASYSEP® D MAGNETIC PARTICLES
Product stable at 2 – 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 22°C), and should be refrigerated upon receipt.
NORMAL RAT SERUM
Product stable at 2 – 8°C until expiry date as indicated on label. Stable for at least 2 years if stored at -20°C. Contents have been sterility tested.
EASYSEP® BLOCKING SOLUTION
Product stable at 2 – 8°C or room temperature (15 - 22°C) until expiry date as indicated on label. Contents have been sterility tested. Please note that repeated exposure to air may cause some crystallization to occur around the edge of the tube. This crystallization does not affect the performance of the blocking solution.

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 实验资料
FACS Profile Results with EasySep® Mouse CD4+CD25+ T Cell Positive Selection Kit

Starting with mouse splenocytes, the CD4+CD25+ cell content of the selected cells typically ranges from 85 - 97%.

Typical EasySep™ Procedure for Mouse Cells


Stemcell 19782 EasySep™ 小鼠CD4+CD25+调节性T细胞分离试剂盒相关产品订购信息
Stemcell  19792  EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit  Immunomagnetic Negative Followed by Positive Selection Kit 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  19752  EasySep™ Mouse CD4+ T Cell Enrichment Kit  Immunomagnetic Negative Selection Kit   
For research use only. Not intended for human or animal diagnostic or therapeutic uses.