产品英文名称：Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit
产品中文名称：Stemcell 19757 EasySep™ 小鼠乳腺干细胞富集试剂盒

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 产品简介
Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit旨在从新鲜解离的小鼠乳腺组织中分离出乳腺干细胞。不需要的细胞被针对非上皮细胞(CD45, CD31, TER119, BP-1)的生物素化的抗体瞄准去除。标记的细胞然后被确认针对抗生物素和葡聚糖的四聚体的抗体配合物识别。这些细胞被绑定到磁性粒子并使用没有柱的EasySep™磁铁分离。未受破坏的上皮细胞富集馏分倾到一个新的管中，然后用CD24和CD49f抗体标记，这样，CD24+CD49f++ 子种群就可以通过荧光激活细胞分选分离。

Stemcell 19757 EasySep™ 小鼠乳腺干细胞富集试剂盒产品组分
Stemcell 19757 EasySep™ 小鼠乳腺干细胞富集试剂盒有五个部分
• EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL
• CD24 Antibody, Clone M1/69, PE-Conjugated, 0.5 mL
• CD49f Antibody, Clone GoH3, FITC-Conjugated, 1.0 mL

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 推荐使用
Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit is designed for the isolation of mouse mammary stem cells from freshly dissociated mammary tissue by negative selection
Stemcell 19757 EasySep™ 小鼠乳腺干细胞富集试剂盒所需设备
• Collagenase/Hyaluronidase Solution (Catalog #07912)
• Gentle Collagenase/Hyaluronidase Solution (Catalog #07919)

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 保存方法
EASYSEP™ MOUSE EPITHELIAL CELL ENRICHMENT COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ BIOTIN SELECTION COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
ANTI-MOUSE CD24-PE ANTIBODY
Product stable at 2 - 8°C until expiry date as indicated on label. Store in the dark. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
ANTI-CD49f-FITC ANTIBODY
Product stable at 2 - 8°C until expiry date as indicated on label. Store in the dark. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.

Stemcell 19757 EasySep™ Mouse Mammary Stem Cell Enrichment Kit 实验资料
FACS Profile Results with EasySep™ Mouse Mammary Stem Cell Enrichment Kit

Freshly dissociated mouse mammary epithelial cells are distributed according to their expression of CD49f and CD24. The mammary repopulating unit (MRU)-rich fraction is found in the CD49f++CD24+ subset. The mammary colony-forming cells (Ma-CFCs) are found in the CD49f+ CD24++ subset. In certain strains of mice (such as C57BI/6J) there can be a small number of CD24++CD49f++ cells (indicated * in the dot plot). Although mammary stem cells have been detected in this fraction, their frequency is much lower than in the MRU fraction.

Typical EasySep™ Procedure for Mouse Cells


Stemcell 19757 EasySep™ 小鼠乳腺干细胞富集试剂盒相关产品订购信息
Stemcell  07912  Collagenase/Hyaluronidase  10X Collagenase/Hyaluronidase in DMEM 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  05610  EpiCult™-B Mouse Medium Kit  Medium for Mouse Mammary Epithelial Cell Culture      
For research use only. Not intended for human or animal diagnostic or therapeutic uses.