Recombinant Human Interleukin-1 beta
规格:
数量:
价格:
折后价:
金牌价格:
联系客服:
英文名称:Recombinant Human Interleukin-1 beta
中文简称:【IL 1 beta】重组人白介素-1β
Recombinant Human Interleukin-1 beta 【IL 1 beta】重组人白介素-1β 细胞因子描述:
白细胞介素-1(interleukin-1,IL-1)是在感染和炎症状态下,由多种细胞产生的、有多方面生物学功能的细胞因子,其生物学作用十分广泛,主要包括参与介导炎症反应、调节免疫、调节机体代谢。IL-1分子重量为17.5KD,有两种类型:IL-1α和IL-1β,主要以IL-1β为主。IL-1受体(interleukin-1 receptor,IL-1R)包括Ⅰ型和Ⅱ型,此外还存在一种内源性IL-1受体拮抗剂 (interleukin-1 receptor antagonist,IL-1ra或IL-1γ),IL-1ra可以与IL-1受体结合,但是不引起相应的作用。炎症情况下,粒细胞巨噬细胞集落刺激因子(GM-CSF) 水平升高,它也能促进IL-1 的上调作用,增加IL-1β的合成;另外关节滑膜细胞也可自分泌IL-1β。
人白介素-1β重组蛋白是在大肠杆菌中重组表达的,是一条非糖基化的多肽链,包含有153个氨基酸,分子量为17000Da。该人白介素-1β重组蛋白最终由标准的色谱技术纯化出。
来源宿主--大肠杆菌中重组表达。
Recombinant Human Interleukin-1 beta 【IL 1 beta】重组人白介素-1β 细胞因子实验须知:
溶解:提供的人白介素-1β (IL 1 beta)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml人白介素-1β (IL 1 beta)的50mM 磷酸缓冲液(pH 7.1)和150mM NaCl溶液中过滤灭菌并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:人白介素-1β (IL 1 beta)冻干粉末在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。
Recombinant Human Interleukin-1 beta 【IL 1 beta】重组人白介素-1β 细胞因子英文描述:
Synonyms
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Introduction
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Description
Interleukin-1 beta Human Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17000 Dalton.
The IL-1b is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 50mM Phosphate buffer pH=7.1 and 150mM NaCl.
Solubility
It is recommended to reconstitute the lyophilized Interleukin-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability (稳定性)
Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity (纯度)
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence (氨基酸序列)
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Val-Arg-Ser.
Biological Activity (生物活性)
The specific activity as determined in the test of augmentation of lymphocyte proliferation assay using mouse thymus was found to be 200,000,000 IU/mg.
Protein content (蛋白质含量)
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.631 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-1 beta Recombinant as a Reference Standard.
OriGen CP-70 CryoPur-DMSO 细胞冻存液 二甲基亚砜 进口USP级 CE认证 CP70
¥1Lifeline LL-0002 VascuLife EnGS Complete Kit 人内皮细胞培养基套装
¥1Anti-Human CD4 PerCP-Cyanine5.5 人CD4抗体
¥4,188Corning 88-571-kit Natural Killer Cell Serum-free Culture Kit II NK细胞活化扩增培养基套装
¥0Cellartis Y50200 MSC Xeno-Free Culture Medium 人间充质干细胞培养基,无外源无需包被
¥1Origen CS250 Freezing Bags vol-max 30-70mL 冻存袋 细胞治疗用药包材
¥1华雅思创生物获谱洛美施品牌授权代理,引领生物医药耗材新趋势
PerkinElmer 华雅现货代理 优势产品!
专门为iPSC 细胞治疗而开发的-康宁 354787 现货
升级您的iPSC细胞治疗研究 —— Corning SyntheGel基质胶系列
【新品】Tecan Revelo™ RNA-Seq High Sensitivity产品 优势 现货
探索SARS-CoV-2的奥秘:Revelo™ RNA-Seq High Sensitivity技术
Countstar Countstar Mira BF在类器官培养中利用
华雅思创生物代理Countstar:专业细胞成像分析设备的领先者
为什么选择康宁G418:细胞培养筛选的黄金标准
探索G418:细胞培养中的重要筛选工具