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ProSpec Recombinant Human Insulin Like Growth Factor-1 【IGF1】重组人胰岛素样生长因子-1

Recombinant Human Insulin Like Growth Factor-1

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  • 保存温度: -18℃
  • 规格型号: 20ug,100ug,1mg
  • 产品编号: HY-001110
  • 产品种类: 特定细胞专用培养基

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英文名称:Recombinant Human Insulin Like Growth Factor-1
中文简称:【IGF1】重组人胰岛素样生长因子-1

 

Recombinant Human Insulin Like Growth Factor-1 【IGF1】重组人胰岛素样生长因子-1 细胞因子产品描述:

胰岛索样生长因子一1(Insulin―like Growth Factor―l,IGF 1)是胰岛素样生长因子的重要成员,与胰岛索高度同源。这个系统的其它成员还包括IGF Ⅱ、1GF―l受体(IGFIR)、IGF Ⅱ受体(IGFIIR)以及4种胰岛素生长因子结合蛋白(IGF BP-1、-2、-3、-4)。其中胰岛素样生长因子-1(Insulin-like growth factor-1,IGF-1)由70个氨基酸组成的单链碱性多肽分子,分子量为7649。它来源于体内许多细胞,通过自分泌、旁分泌和内分泌机制并与IGF-1受体(IGF-1R)结合发挥多种生理作用。作为一类促进细胞生长、具有胰岛素样代谢效应的因子。IGF-1受营养状态、激素、遗传等多因素的调节,在心血管疾病、内分泌代谢病及肿瘤等的病理生理过程中发挥重要作用。
人胰岛素样生长因子-1重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有70个氨基酸,分子量为7655Da。该人胰岛素样生长因子-1重组蛋白最终由标准的色谱技术纯化出。
来源宿主---大肠杆菌中重组表达。

 

Recombinant Human Insulin Like Growth Factor-1 【IGF1】重组人胰岛素样生长因子-1 细胞因子产品实验须知:

溶解:提供的人胰岛素样生长因子-1(IGF1)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含人胰岛素样生长因子-1(IGF1)的溶液(不含添加剂)中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:人胰岛素样生长因子-1(IGF1)冻干粉末在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。

 

Recombinant Human Insulin Like Growth Factor-1 【IGF1】重组人胰岛素样生长因子-1 细胞因子产品英文描述:

Synonyms 

Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA, Mechano growth factor, MGF.


Introduction 

The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).


Description 

Insulin-Like Growth Factor-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.7kDa.
IGF-I is purified by proprietary chromatographic techniques.


Source 

Escherichia Coli.


Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation 

The protein was lyophilized without any additives.


Solubility 

It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


Stability 

Lyophilized Insulin-Like Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.


Purity 

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.


Amino acid sequence 

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.


Biological Activity 

The activity as determined by the dose-dependent proliferation of mouse FDC-P1 is less than 1.0 ng/ml, corresponding to a specific activity of 1x106 units/mg.


Protein content 

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.502 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IGF1 as a Reference Standard.

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