Recombinant Human Fibroblast Growth Factor-basic
英文名称：Recombinant Human Fibroblast Growth Factor-basic
Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重组人碱性成纤维细胞生长因子 产品描述：
碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）是成纤维细胞生长因子蛋白（fibroblast growth factors，FGFs）家族中的一员。目前已发现至少22种FGF，其中N3F-1（aFGF）、FGF-2（bFGF）及FGF-7的研究较为深入。bFGF是哺乳动物和人体中一种非常微量的活性物质，具有广泛的生理功能和重要的临床应用价值。bFGF不仅能刺激新生血管形成，参与创伤愈合和组织再生，促进胚胎组织发育和分化等，此外还与肿瘤的发生发展密切相关。近年来，重组bFGF已被应用于临床治疗创伤、溃疡及神经系统等疾病。
Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重组人碱性成纤维细胞生长因子 产品实验须知：
溶解：提供的人碱性成纤维细胞生长因子(FGF 2)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人碱性成纤维细胞生长因子(FGF 2)的PBS（pH 7.4）溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。
保存：人碱性成纤维细胞生长因子(FGF 2)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。
Recombinant Human Fibroblast Growth Factor-basic 【FGF 2】重组人碱性成纤维细胞生长因子 产品英文描述：
Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton.
The FGF-b is purified by proprietary chromatographic techniques.
Sterile Filtered White lyophilized (freeze-dried) powder.
The protein was lyophilized from a concentrated (1mg/ml) solution in PBS, pH 7.4.
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 96.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.
Biological Activity (生物活性）
The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is <0.5 ng/ml, corresponding to a specific activity of 2,000,000 Units/mg.
Protein content （蛋白质含量）
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard.
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