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ProSpec Recombinant Human Vascular Endothelial Growth Factor (121),Sf9 【VEGF (121a.a.) Sf9】重组人血管内皮生长因子(121),Sf9表达

Recombinant Human Vascular Endothelial Growth Factor (121),Sf9

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  • 生产厂家: ProSpec
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  • 保存温度: -18℃
  • 规格型号: 2ug,10ug,1mg
  • 产品编号: HY-001114
  • 产品种类: 特定细胞专用培养基

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英文名称:Recombinant Human Vascular Endothelial Growth Factor (121),Sf9
中文简称:【VEGF (121a.a.) Sf9】重组人血管内皮生长因子(121),Sf9表达

 

Recombinant Human Vascular Endothelial Growth Factor (121),Sf9 【VEGF (121a.a.) Sf9】重组人血管内皮生长因子(121),Sf9表达  细胞因子产品描述

血管内皮生长因子(Vascular endothelial growth factor,VEGF)是血管内皮细胞特异性的肝素结合生长因子(heparin-binding growth factor),可在体内诱导血管新生。VEGF有六种不同亚型,包括VEGF-A,-B,-C,-D,及-E;其分子量从35至44kDa不等,每个亚型特异性地与三个“血管内皮生长因子受体”(VEGFR-1,-2,及-3)的特定组合相结合。VEGF是高度保守的同源二聚体糖蛋白。二条分子量各为24kDa的单链以二硫键组成二聚体。VEGF分解的单体无活性,去除N 2糖基对生物效应无影响,但可能在细胞分泌中起作用。由于mRNA不同的剪切方式,产生出分别VEGF121、VEGF145、VEGF165、VEGF185、VEGF206等至少5种蛋白形式,其中VEGF121、VEGF145、VEGF165是分泌型可溶性蛋白,能直接作用于血管内皮细胞促进血管内皮细胞增殖,增加血管通透性。
人血管内皮生长因子(121),Sf9表达重组蛋白是在杆状病毒-昆虫Sf9细胞中重组表达的,是由两条糖基化多肽链构成的同源二聚体。包含有121个氨基酸残基,分子量为36 kDa。与其它形式的人血管内皮生长因子相比,人血管内皮生长因子(121)循环更快,与血管硫酸肝素结合更紧密。该人血管内皮生长因子(121),Sf9表达重组蛋白最终由标准的色谱技术纯化出。
来源宿主---杆状病毒-昆虫Sf9细胞中重组表达.

 

Recombinant Human Vascular Endothelial Growth Factor (121),Sf9 【VEGF (121a.a.) Sf9】重组人血管内皮生长因子(121),Sf9表达 细胞因子产品实验须知:

溶解:提供的人血管内皮生长因子(121),Sf9表达(VEGF (121a.a.) Sf9)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml人血管内皮生长因子(121),Sf9表达(VEGF (121a.a.) Sf9)的溶液(不含添加剂)中冻干产生的。冻干的人血管内皮生长因子具有非常好的水溶性。我们建议加PBS或培养液 (0.1% HSA或BSA载体蛋白)以配制工作储存液到不低于50ug/ml。
保存:人血管内皮生长因子(121),Sf9表达(VEGF (121a.a.) Sf9)在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。

 

Recombinant Human Vascular Endothelial Growth Factor (121),Sf9 【VEGF (121a.a.) Sf9】重组人血管内皮生长因子(121),Sf9表达 细胞因子产品英文描述:

Synonyms 

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.


Introduction 

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g. stimulation monocyte/ macrophagemigration, neurons, cancer cells, kidney epithelial cells ).VEGF mediates increased vascular permeability, induces angiogenesis, vasculogenesis and endothelial cell growth, promotes cell migration, and inhibits apoptosis. In vitro, VEGF has been shown to stimulate endothelial cell mitogenesisand cell migration. VEGF is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor.
Elevated levels of this protein are linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy.


Description 

Vascular Endothelial Growth Factor-121 Human Recombinant produced in insect cells as an 18kDa homodimer, is a glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of approximately 36kDa.
VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
The VEGF-121 is purified by proprietary chromatographic techniques.

Source 

Sf9, Insect Cells.


Physical Appearance 

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation 

The protein was lyophilized from a solution containing 50mM acetic acid.


Solubility 

The lyophilized VEGF121 should be reconstituted in 50mM acetic acid to a concentration not lower than 50µg/ml.


Stability 

Lyophilized Vascular Endothelial Growth Factor 121 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-121 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.


Purity 

Greater than 95.0% as determined by SDS-PAGE.


Biological Activity 

The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF121 has been determined to be in the range of 1-4 ng/ml.
Amino Acid Sequence 

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECV
PTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQEKCDKPR.


Usage 

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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