Recombinant Human Tumor Necrosis Factor-alpha
规格:
数量:
价格:
折后价:
金牌价格:
联系客服:
英文名称:Recombinant Human Tumor Necrosis Factor-alpha
中文简称:【TNF a 】重组人肿瘤坏死因子α
Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重组人肿瘤坏死因子α 细胞因子产品描述:
肿瘤坏死因子-α(Tumor Necrosis Factor-alpha,TNF-α)是一种主要由单核-吞噬细胞产生的单核因子,不仅具有选择性地杀伤某些肿瘤细胞,而且有多种免疫调节作用。生理条件下具有生物学活性的人肿瘤坏死因子α(hTNF-α)是一个紧密的三聚体,其活性位点位于两个相邻亚基之间的V型结构域,hTNF-α有两种不同的受体,分别介导不同的生物学活性,在对hTNF-α结构与功能研究的基础上,人们对其N末端、C末端和p75受体结合位点进行基因改造。研制了一系列衍生物,以提高hTNF-α的抗肿瘤活性,降低毒副反应。
人肿瘤坏死因子α重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有158个氨基酸,分子量为17483.77Da。该人肿瘤坏死因子α重组蛋白最终由标准的色谱技术纯化出。
来源宿主---大肠杆菌中重组表达
Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重组人肿瘤坏死因子α 细胞因子产品使用须知:
溶解:提供的人肿瘤坏死因子α(TNF a)产品为过滤灭菌的白色冻干粉末;该冻干粉末中每毫克人肿瘤坏死因子是从20mM PBS(pH 7.2),10mM NaCl溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:人肿瘤坏死因子α(TNF a )在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。
Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重组人肿瘤坏死因子α 细胞因子产品英文描述:
Synonyms
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Introduction
Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
Description
Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and having a molecular mass of 17.5kDa.
The TNF-alpha is purified by standard chromatographic techniques.
Source (来源)
Escherichia Coli.
Physical Appearance (外观)
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
1mg of TNF-alpha Human contain 20mM PB, pH-7.2, and 100mM NaCl.
Solubility (溶解度)
It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability (稳定性)
Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity (纯度)
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence (氨基酸序列)
MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.
Biological Activity (生物活性)
The Specific Activity is >5.0×107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.
OriGen CP-70 CryoPur-DMSO 细胞冻存液 二甲基亚砜 进口USP级 CE认证 CP70
¥1Lifeline LL-0002 VascuLife EnGS Complete Kit 人内皮细胞培养基套装
¥1Anti-Human CD4 PerCP-Cyanine5.5 人CD4抗体
¥4,188Corning 88-571-kit Natural Killer Cell Serum-free Culture Kit II NK细胞活化扩增培养基套装
¥0Cellartis Y50200 MSC Xeno-Free Culture Medium 人间充质干细胞培养基,无外源无需包被
¥1Origen CS250 Freezing Bags vol-max 30-70mL 冻存袋 细胞治疗用药包材
¥1【爆款推荐】适合再生医疗研究用的人间充质干细胞培养基
【专利热卖产品】Takara Cellartis iPS定向分化肝脏细胞专利技术解析
人间充质干细胞培养基怎么选,看这三个点就可以了!
人胚胎干细胞(ESC)/诱导多能干细胞(iPSC)培养推荐:Cellartis® DEF-CS™系列产品 授权代理
【CRISPR/Cas9系统】人iPS细胞推荐基因编辑工具
为什么说PAXgene是RNA保存必选产品,数据来说话!
RNA转录组学研究对临床医学的重大意义【附带RNA样本保存神器】
PLTGOLD500GMP人血小板裂解物,为细胞培养和药物生产提供最佳环境
要养支气管上皮细胞?Lifeline上皮细胞培养基助您一臂之力
分析RNA降解的常见原因,如何防止RNA降解