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ProSpec Recombinant Human Interleukin-4 CHO 【IL 4 CHO】重组人白介素-4,CHO表达

Recombinant Human Interleukin-4 CHO

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  • 生产厂家: ProSpec
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  • 保存温度: -18℃
  • 规格型号: 2ug,10ug,1mg
  • 产品编号: HY-001130
  • 产品种类: 特定细胞专用培养基

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英文名称:ecombinant Human Interleukin-4 CHO
中问简称:【IL 4 CHO】重组人白介素-4,CHO表达

 

Recombinant Human Interleukin-4 CHO 【IL 4 CHO】重组人白介素-4,CHO表达 细胞因子描述:

IL4是多向性的激活T细胞产生的细胞因子。IL4是白介素4受体的配体。IL13白介素4受体也绑定,这可能导致许多重叠的细胞因子和IL13功能。STAT6、传感器信号和转录激活剂,已被证明在介导免疫中发挥核心作用的监管这一细胞因子的信号。这种基因,IL3 IL5、IL13 CSF2形成一个细胞因子基因簇5号染色体上q,特别是接近IL13基因。IL4,IL13 IL5发现监管协调由几个远程监管元素在染色体上的120多千碱基范围。两个或者拼接成绩单变异的基因编码已报告不同的亚型。
人白介素-4,CHO表达重组蛋白是在CHO中国仓鼠卵巢细胞中重组表达的,是一条单链、非糖基化的多肽链,包含有129个氨基酸,分子量为14kDa,由于糖基化而导致在SDS-PAGE电泳显示分子量为18 kDa。该人白介素-4,CHO表达重组蛋白最终由标准的色谱技术纯化出。
来源宿主---CHO中国仓鼠卵巢细胞中重组表达。

 

Recombinant Human Interleukin-4 CHO 【IL 4 CHO】重组人白介素-4,CHO表达 细胞因子使用须知:

溶解:提供的人白介素-4,CHO表达(IL 4 CHO)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含人白介素-4,CHO表达(IL 4 CHO)的磷酸盐缓冲盐水(pH 7.4)中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:人白介素-4,CHO表达(IL 4 CHO)冻干粉末在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。

 

Recombinant Human Interleukin-4 CHO 【IL 4 CHO】重组人白介素-4,CHO表达 细胞因子英文描述:

Synonyms 

BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.


Introductionn 

IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.


Description 

Interleukin-4 Human Recombinant produced in CHO is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14 kDa, the glycosilation of IL-4 migrates as 18 kDa on SDS-PAGE.
The IL-4 is purified by proprietary chromatographic techniques.


Source 

Chinese Hamster Ovarian Cells.


Physical Appearance
  (外观)

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation 

Lyophilized from a Phosphate- Buffered Saline (pH 7.4).


Solubility  (溶解性)

It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


Stability     (稳定性)

Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.


Purity     (纯度)

Greater than 97.0% as determined by

(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.


Biological Activity   (生物活性)

Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2ng/ml corresponding to a Specific Activity of 5,000,000-20,000,000IU/mg.

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