Recombinant Human Glial-Derived Neurotrophic Factor
英文名称：Recombinant Human Glial-Derived Neurotrophic Factor
Recombinant Human Glial-Derived Neurotrophic Factor 【GDNF】重组人神经胶质源神经营养因子 细胞因子产品描述：
GDNF promotes the survival and differentiation of dopaminergic neurons in culture，and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF，two additional alternative transcripts encoding distinct proteins，referred to as astrocyte-derived trophic factors，have also been described. Mutations in this gene may be associated with Hirschsprung disease.
GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
人神经胶质源神经营养因子重组蛋白是在大肠杆菌中重组表达的，是由两条非糖基化多肽链组成的同源二聚体。该重组蛋白包含有2 x 135个氨基酸，总分子量为30，360Da。该人神经胶质源神经营养因子重组蛋白最终由标准的色谱技术纯化出。
Recombinant Human Glial-Derived Neurotrophic Factor 【GDNF】重组人神经胶质源神经营养因子 细胞因子产品使用须知：
溶解： 提供的人神经胶质源神经营养因子 (GDNF)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含人神经胶质源神经营养因子 (GDNF)的10mM柠檬酸钠和150mM NaCl溶液中经透析并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。
保存：人神经胶质源神经营养因子 (GDNF)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。
Recombinant Human Glial-Derived Neurotrophic Factor 【GDNF】重组人神经胶质源神经营养因子 细胞因子产品英文概述：
Glial derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton.
GDNF is purified by proprietary chromatographic techniques.
Physical Appearance （外观）
Sterile Filtered White lyophilized (freeze-dried) powder.
GDNF was lyophilized after dialysis against 10mM sodium citrate and 150mM NaCl.
It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Greater than 98.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence （氨基酸序列）
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.
Biological Activity （生物活性）
The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000Units/mg.
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Cellartis Y50200 MSC Xeno-Free Culture Medium 人间充质干细胞培养基，无外源无需包被￥1
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