Recombinant Mouse Interferon-gamma
规格:
数量:
价格:
折后价:
金牌价格:
联系客服:
英文名称:Recombinant Mouse Interferon-gamma
中文简称:【mIFN g】重组小鼠干扰素-γ
Recombinant Mouse Interferon-gamma 【mIFN g】重组小鼠干扰素-γ 细胞因子产品描述:
干扰素(interferon,IFN)是细胞对相关刺激反应时所产生的细胞信号蛋白质,是细胞因子超家族中一个糖蛋白类同源细胞因子家族,称为IFN家族。已鉴定出该家族中的3个主要成员:IFN-α、IFN-β和IFN-γ。其中干扰素-γ(Interferon-gamma,IFN-γ)是一种具有抗病毒、抗肿瘤和免疫调节作用的细胞因子,主要由活化的T细胞和NK细胞产生,在机体免疫系统中发挥重要作用。IFN-γ是一类分泌型糖蛋白,由143个氨基酸组成,相对分子量是16924。而重组人干扰素-γ相对分子量在14000-16000之间,这是由于重组IFN-γ在C末端有氨基酸丢失现象。
小鼠干扰素-γ重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有134个氨基酸,分子量为15601Da。该小鼠干扰素-γ重组蛋白最终由标准的色谱技术纯化出。
来源宿主—大肠杆菌中重组表达
Synonyms
Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.
Introduction
IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Description
Interferon-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.
Source (来源)
Escherichia Coli.
Physical Appearance (物理特征)
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4 and 5% trehalose.
Solubility
It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability (稳定性)
Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity (纯度)
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence (氨基酸序列)
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.
Biological Activity (生物活性)
The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 Units/mg.
Recombinant Mouse Interferon-gamma 【mIFN g】重组小鼠干扰素-γ 细胞因子产品使用须知:
溶解:提供的小鼠干扰素-γ(mIFN g)产品为经0.2um孔径过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml小鼠干扰素-γ(mIFN g)的PBS(pH 7.4)溶液中过滤灭菌并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:小鼠干扰素-γ(mIFN g)冻干粉末在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。
OriGen CP-70 CryoPur-DMSO 细胞冻存液 二甲基亚砜 进口USP级 CE认证 CP70
¥1Lifeline LL-0002 VascuLife EnGS Complete Kit 人内皮细胞培养基套装
¥1Anti-Human CD4 PerCP-Cyanine5.5 人CD4抗体
¥4,188Corning 88-571-kit Natural Killer Cell Serum-free Culture Kit II NK细胞活化扩增培养基套装
¥0Cellartis Y50200 MSC Xeno-Free Culture Medium 人间充质干细胞培养基,无外源无需包被
¥1Origen CS250 Freezing Bags vol-max 30-70mL 冻存袋 细胞治疗用药包材
¥1华雅思创生物获谱洛美施品牌授权代理,引领生物医药耗材新趋势
PerkinElmer 华雅现货代理 优势产品!
专门为iPSC 细胞治疗而开发的-康宁 354787 现货
升级您的iPSC细胞治疗研究 —— Corning SyntheGel基质胶系列
【新品】Tecan Revelo™ RNA-Seq High Sensitivity产品 优势 现货
探索SARS-CoV-2的奥秘:Revelo™ RNA-Seq High Sensitivity技术
Countstar Countstar Mira BF在类器官培养中利用
华雅思创生物代理Countstar:专业细胞成像分析设备的领先者
为什么选择康宁G418:细胞培养筛选的黄金标准
探索G418:细胞培养中的重要筛选工具