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ProSpec CYT-358 Recombinant Mouse Interferon-gamma【mIFN g】重组小鼠干扰素-γ

Recombinant Mouse Interferon-gamma

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  • 注册品牌: ProSpec
  • 生产厂家: ProSpec
  • 订购货号: CYT-358
  • 保存温度: -18℃
  • 规格型号: 20ug,100ug,1mg
  • 产品编号: HY-001137
  • 产品种类: 特定细胞专用培养基

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英文名称:Recombinant Mouse Interferon-gamma
中文简称:【mIFN g】重组小鼠干扰素-γ

 

Recombinant Mouse Interferon-gamma 【mIFN g】重组小鼠干扰素-γ 细胞因子产品描述:

干扰素(interferon,IFN)是细胞对相关刺激反应时所产生的细胞信号蛋白质,是细胞因子超家族中一个糖蛋白类同源细胞因子家族,称为IFN家族。已鉴定出该家族中的3个主要成员:IFN-α、IFN-β和IFN-γ。其中干扰素-γ(Interferon-gamma,IFN-γ)是一种具有抗病毒、抗肿瘤和免疫调节作用的细胞因子,主要由活化的T细胞和NK细胞产生,在机体免疫系统中发挥重要作用。IFN-γ是一类分泌型糖蛋白,由143个氨基酸组成,相对分子量是16924。而重组人干扰素-γ相对分子量在14000-16000之间,这是由于重组IFN-γ在C末端有氨基酸丢失现象。
小鼠干扰素-γ重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有134个氨基酸,分子量为15601Da。该小鼠干扰素-γ重组蛋白最终由标准的色谱技术纯化出。
来源宿主—大肠杆菌中重组表达

 

Synonyms 

Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.


Introduction
 

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.
IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.


Description 

Interferon-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.
The IFN-gamma is purified by proprietary chromatographic techniques.


Source   (来源)

Escherichia Coli.


Physical Appearance
  (物理特征)

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation 

Lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4 and 5% trehalose.


Solubility 

It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


Stability  (稳定性)

Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.


Purity   (纯度)

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.


Amino acid sequence  (氨基酸序列)

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.


Biological Activity  (生物活性)

The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 Units/mg.

 

Recombinant Mouse Interferon-gamma 【mIFN g】重组小鼠干扰素-γ 细胞因子产品使用须知:
溶解:提供的小鼠干扰素-γ(mIFN g)产品为经0.2um孔径过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml小鼠干扰素-γ(mIFN g)的PBS(pH 7.4)溶液中过滤灭菌并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:小鼠干扰素-γ(mIFN g)冻干粉末在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。

 

 

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