Recombinant Human Bone Morphogenetic protein-4
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英文名称:Recombinant Human Bone Morphogenetic protein-4
中文简称:【BMP 4】重组人骨形态发生蛋白-4
Recombinant Human Bone Morphogenetic protein-4 【BMP 4】重组人骨形态发生蛋白-4 细胞因子产品描述:
骨形态发生蛋白(Bone Morphogenetic Protein,BMP)属转化生长因子超家族成员,这种蛋白质具有使未分化间充质定向分化为成骨细胞,并进而合成胶原、形成钙化骨组织能力,因此命名为骨形态发生蛋白。BMP-4作为BMPs家族成员之一,在空间结构上与BMPs大体结构有一致性,只有在成熟区序列方面有其特异性。BMP是一种二聚体分子,由两个单体以一个二硫键结合而成。它首先被合成为较大前体蛋白,前体含一个100-125个氨基酸组成羧基末端。通过蛋白质分解过程释放后,两个前体以二硫键结合发生二聚作用,有活性同二聚体或异二聚体被分泌出来。BMP-4氨基末端含有一段信号肽序列,且含有4个可能氨基末端连接糖基化位点,其中3个在同一位置。
人骨形态发生蛋白-4重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有116个氨基酸,分子量为13009Da。该人骨形态发生蛋白-4重组蛋白最终由标准的色谱技术纯化出。
来源宿主---大肠杆菌中重组表达
Recombinant Human Bone Morphogenetic protein-4 【BMP 4】重组人骨形态发生蛋白-4 细胞因子产品使用须知:
溶解:提供的人骨形态发生蛋白-4(BMP 4)产品为过滤灭菌的白色冻干粉末;该冻干粉末是从含1mg/ml人骨形态发生蛋白-4(BMP 4)的溶液(不含添加剂)中冻干产生的。我们建议加无菌的20mM AcOH(乙酸)以配制工作储存液到不低于100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:人骨形态发生蛋白-4(BMP 4)冻干粉末在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。
Recombinant Human Bone Morphogenetic protein-4 【BMP 4】重组人骨形态发生蛋白-4 细胞因子产品英文描述:
Synonyms
BMP4, ZYME, BMP2B, BMP2B1.
Introduction
The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
Description
Bone Morphogenetic Protein-4 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, Polypeptide chain containing 116 amino acids and having a molecular mass of 13kDa.
The BMP-4 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance (物理特征)
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
BMP-4 was lyophilized from a 0.2?m filtered concentrated (1mg/ml) solution in 20mM Na2CO3 buffer, pH 9.0.
Solubility It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-4 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability (稳定性)
Lyophilized Bone Morphogenetic Protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity (纯度)
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence (氨基酸序列)
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR.
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