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产品编号:HY-001328

STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR™1(产品号 #05850)/mTeSR™2 (产品号#05860)

mTeSR™1

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  • 注册品牌: STEMCELL
  • 生产厂家: 美国stemrd公司
  • 订购货号:
  • 保存温度: 2~8℃,-20℃
  • 规格型号: 500ml/Kit
  • 产品编号: HY-001328
  • 产品种类: 蛋白激酶(PKA,PKC)

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产品英文名称:mTeSR™1
产品中文名称:iPSC/ESC无血清培养基

 

STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR™1(产品号 #05850)/mTeSR™2 (产品号#05860)产品简介:
STEMCELL Technologies 研发的mTeSR™1(产品号 #05850)和 mTeSR™2(产品号#05860),作为无饲养层细胞条件下维持 hPSC 的标准化培养基。它们的成分基于 Ludwig等公布的研究成果,是完全的、成分确定的无血清培养基,其生产得到 WiCell Research Institute 的授权。mTeSR?1含牛血清白蛋白来源,用于支持长期、无饲养层细胞条件下培养 hPSC 以及在无饲养层细胞条件下衍生 hiPSC。TeSR?2则是不含动物蛋白的配方,同样支持长期、无饲养层细胞条件下培养 hPSC。两者均不需要进一步添加生长因子。它们旨在维持和扩增未分化的hPSC,并与作为基质的 BD Matrigel? hESC-qualified Matrix(BD 产品号#354277)配合使用。STEMCELL Technologies 预先对每一批次的 BD Matrigel?的质量进行检测,确保均一性、可重复性和可靠性。若要获得完全不含动物蛋白的培养体系,TeSR?2 可与Vitronectin第替代基质配合使用。
在 mTeSR™1 和 TeSR™2 中培养的 hPSC:
·  表型均匀且核型正常
·  表达高水平的多能性标记如:Oct-3/4、SSEA-3、SSEA-4、TRA-1-60、TRA-1-81
·  表达多能性所需的基因:Oct-3/4、nanog
·  形成包含内、中、外胚层谱系衍生物的畸胎瘤
·  可在体外分化为功能成熟的细胞类型(如造血细胞)
·  从基于饲养层细胞的培养物转化时,不需要驯化培养期(即无细胞低产期)
使用 mTeSR™1 和 TeSR™2 的优点包括:
·  去除不确定的培养基成分和消除与饲养层细胞和条件培养基有关的固有可变性,使 hPSC 培养的条件保持一致性
·  标准化培养方法,从而提高数据的可重复性
·  省去制备饲养层细胞或条件培养基的程序,节省了时间和劳力
·  完全的培养基配方,无需添加额外的生长因子或其他添加物

STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR™1(产品号 #05850)/mTeSR™2 (产品号#05860)相关产品订购信息:
Product Name  Description  Catalog #  Size  Price  Quantity
mTeSR?1  Defined, feeder-free maintenance medium for human ESCs and iPSCs  05850  500 mL Kit  Request pricing
mTeSR?1  Defined, feeder-free maintenance medium for hESCs and hiPSCs, 1 L kit  05857  1 L Kit  Request pricing
mTeSR?1  Defined, feeder-free maintenance medium for hESCs and hiPSCs, 10 pack  05870  10 Kits  Request pricing
mTeSR?1  Defined, feeder-free maintenance medium for hESCs and hiPSCs, 25 pack  05875  25 Kits  Request pricing
STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR?1(产品号 #05850)/TeSR?2 (产品号#05860)英文描述:
mTeSR™1 is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain hundreds of ES cell and iPS cell lines in over 40 countries, and has supported top pluripotent stem cell publications and researchers.
mTeSR™1 is a highly specialized, serum-free and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.


STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR™1(产品号 #05850)/mTeSR™2 (产品号#05860)其他相关产品订购信息:

Product Name  Description                                                                                                                                                                                         Catalog #
TeSR™2  Animal protein-free, defined, feeder-independent medium for maintenance of undifferentiated human ES and iPS cells  05860
AggreWell™  Simple and Standardized Production of Embryoid Bodies  27845
mFreSR™  Defined, Serum-Free Cryopreservation Medium for Human ES cells and iPS cells  05854
CryoStor® CS10  Animal Component-Free, Defined Cryopreservation Medium  07930
EasySep™ hESC-Derived CD34 Positive Selection Kit  Immunomagnetic Positive Selection Kit  18167
Mouse IgG (H+L) Antibody, FITC-Conjugated  Goat Polyclonal Antibody to Mouse IgG (H+L) – FITC Conjugated  10210
IgM Antibody, FITC-Conjugated  Goat Polyclonal Antibody to Mouse IgM – FITC Conjugated  10211
IgM Antibody, APC-Conjugated  Goat Polyclonal Antibody to Rat IgM – APC Conjugated  10215
ACCUTASE™  ACCUTASE™ Cell Detachment Solution  07920
Dispase (1 U/mL)  Dispase 1 U/mL  07923
STEMdiff™ APEL™ Medium  Serum-Free, Animal Component-Free Medium for Differentiation of Human ES and iPS cells to Multiple Lineage Types  05210
StemAdhere™ Defined Matrix for hPSC  For maintenance of human ES and iPS cells in combination with mTeSR™1 or TeSR™2  07160
Anti-Sox-2 Antibody  Rabbit Polyclonal Antibody Against Human Sox-2  60055
Anti-Mouse SSEA-1 (CD15) Antibody, Clone MC-480  Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-1 (CD15)  60060
Anti-Mouse SSEA-3 Antibody, Clone MC-631  Rat Monoclonal Antibody Against Human, Mouse, Rat SSEA-3  60061
Anti-Human SSEA-4 Antibody, Clone MC-813-70  Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-4  60062
Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R  Mouse Monoclonal Antibody Against Human, Rhesus, Rabbit TRA-1-60 (Podocalyxin)  60064
Anti-Human TRA-1-81 Antibody, Clone TRA-1-81  Mouse Monoclonal Antibody Against Human, Rat, Rhesus TRA-1-81  60065
Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E  Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-49 (Tissue Non-Specific Alkaline Phosphatase)  60066
Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J  Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-54 (Tissue Non-Specific Alkaline Phosphatase)  60067
Mouse IgM, kappa Isotype Control Antibody, Clone MM-30  Mouse Monoclonal IgM, kappa Isotype Control Antibody  60069
Gentle Cell Dissociation Reagent  Enzyme-Free Cell Dissociation Reagent  07174
STEMdiff™ Definitive Endoderm Kit  Fully Defined Animal Component-Free Medium for the Differentiation of Human ES and iPS Cells to Definitive Endoderm.  05110
Oct-3/4 Antibody, Clone 40  Mouse Monoclonal Antibody to Oct-3/4  01550
Vitronectin XF™  Chemically Defined, Xeno-Free Cell Attachment Factor that Supports the Growth and Differentiation of Human Pluripotent Stem Cells Under Serum-Free, Feeder-Free Conditions  07180
STEMdiff™ Neural Induction Medium  Defined, Serum-Free Induction Medium for Neural Differentiation of Human ES and iPS Cells

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