Human RANTES Pre-Coated ELISA kit
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RANTES是CC趋化因子,可通过CCR1,CCR3,CCR5和US28(巨细胞病毒受体)受体传导信号。 它是一种趋化单核细胞,记忆T细胞(CD4 + / CD45RO),嗜碱性粒细胞和嗜酸性粒细胞的趋化剂。RANTES还具有抑制某些HIV-1,HIV-2和猿免疫缺陷病毒(SIV)的能力。
BioGems人类RANTES预包被ELISA(酶联免疫吸附测定)试剂盒采用固相免疫测定法,专门设计用于检测RANTES,96孔板预包被RANTES特异性小鼠单克隆抗体。它含有大肠杆菌表达的重组RANTES。检测抗体是RANTES特异性的生物素化山羊多克隆抗体。该试剂盒已经过分析验证,是即用型试剂。
 
 
Product Details
| Assay Type: | Solid Phase Sandwich ELISA: 96-Well Strip Plate | 
| Sensitivity: | <3pg/ml | 
| Range: | 15.6pg/ml-1000pg/ml | 
| Specificity: | Natural and recombinant Human Rantes | 
| Uniprot: | D0EI67 | 
| Interference: | No detectable cross-reactivity with other relevant proteins | 
| Storage Conditions: | Store the unopened product at 2 – 8 ℃. | 
Components
| Description | Quantity | Volume | 
| Anti-Human Rantes Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells | 
| Human Rantes Standard | 2 | 10 ng vial | 
| Human Rantes Biotinylated antibody (100x) | 1 | 130 µL | 
| Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL | 
| Sample Diluent | 1 | 30mL | 
| Antibody Diluent | 1 | 12mL | 
| Avidin-Biotin-Peroxidase Diluent | 1 | 12mL | 
| Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack | 
| Color Developing Reagent | 1 | 10mL | 
| Stop Solution | 1 | 10mL | 
| Plate Sealers | 4 | Each | 
Reproducibility
| 
 | Intra-Assay | Inter-Assay | ||||
| Sample ID | 1 | 2 | 3 | 1 | 2 | 3 | 
| n= | 16 | 16 | 16 | 24 | 24 | 24 | 
| Mean Measured Concentration (pg/mL) | 147 | 293 | 501 | 110 | 339 | 658 | 
| Standard Deviation | 5.88 | 15.53 | 31.1 | 8.14 | 24.41 | 56.6 | 
| Consistency (%CV) | 4 | 5.3 | 6.2 | 7.4 | 7.2 | 8.6 | 
Description
RANTES is a CC chemokine that can signal through the CCR1, CCR3, CCR5 and US28 (cytomegalovirus receptor) receptors. It is a chemoattractant towards monocytes, memory T cells (CD4+/CD45RO), basophils, and eosinophils. RANTES also has the capability to inhibit certain strains of HIV-1, HIV-2 and simian immunodeficiency virus (SIV).
The BioGems Human RANTES Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure RANTES with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for RANTES. It contains E. coli-expressed recombinant RANTES. The detection antibody is a biotinylated goat polyclonal antibody specific for RANTES. The kit is analytically validated with ready to use reagents.
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