Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics
规格:
数量:
价格:
折后价:
金牌价格:
联系客服:
Cellartis DEF-CS 500 iPS无外源3D悬浮培养基
传统的2D培养系统无法支持放大培养(Scale-up)所需细胞数量级别(> 109个细胞/处理)以维持细胞疗法的增长需求。 3D悬浮培养是大规模生产多能细胞的可行经济有效的解决方案。尽管干细胞传统上难以适应悬浮培养,但CellArtis研究人员已经开发出一种培养体系,可以在成分确定、无饲养层和无异源物条件下实现稳定高效的生长并维持多能性。Cellartis DEF-CS 500 iPS无外源3D悬浮培养基与生物反应器系统配套,为创建大量高质量,未分化的起始材料提供了理想的方法。该培养基的配方基于2D培养基Cellartis DEF-CS 500 Xeno-Free Culture Medium,2D培养的细胞可以轻松过渡到3D以实现可靠的放大。
英文名称:Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics
中文名称:Cellartis DEF-CS 500无外源3D悬浮iPS培养基,不含抗生素
用途:iPS 细胞3D悬浮细胞治疗所需10亿级
保存:基础培养基2-8°C,添加剂-20°C
组份
基础培养基+三种添加剂
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)
500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No Y30046; not sold separately)
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)
o 500 µl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)
o 200 µl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)
o 400 µl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)
自行购买试剂
缓冲液和细胞分离试剂
PBS Dulbecco’s w/o Ca2+ & Mg2+ (D-PBS –/–)
Accumax (Merck Millipore, Cat. No. SCR006)
可选抗生素和消化试剂
Gentamicin (Thermo Fisher, Cat. No. 15750)
Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)
细胞培养参数
细胞接种量 |
锥形培养瓶大小 |
培养体积 |
1–4 x 106 |
25 ml |
5–15 ml |
3–8 x 106 |
100 ml |
16–30 ml |
7–20 x 106 |
250 ml |
35–65 ml |
Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium
Traditional 2D culture systems simply cannot support the scale-up required (>109 cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions. The Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium paired with a perfusion bioreactor system provides an ideal method for creating large amounts of high-quality, undifferentiated starting material. The formulation of this medium is based on the 2D medium, Cellartis DEF-CS 500 Xeno-Free Culture Medium, and cells growing in 2D can easily be transitioned to 3D for robust scale-up.
OriGen CP-70 CryoPur-DMSO 细胞冻存液 二甲基亚砜 进口USP级 CE认证 CP70
¥1Lifeline LL-0002 VascuLife EnGS Complete Kit 人内皮细胞培养基套装
¥1Anti-Human CD4 PerCP-Cyanine5.5 人CD4抗体
¥4,188Corning 88-571-kit Natural Killer Cell Serum-free Culture Kit II NK细胞活化扩增培养基套装
¥0Cellartis Y50200 MSC Xeno-Free Culture Medium 人间充质干细胞培养基,无外源无需包被
¥1Origen CS250 Freezing Bags vol-max 30-70mL 冻存袋 细胞治疗用药包材
¥1华雅思创生物获谱洛美施品牌授权代理,引领生物医药耗材新趋势
PerkinElmer 华雅现货代理 优势产品!
专门为iPSC 细胞治疗而开发的-康宁 354787 现货
升级您的iPSC细胞治疗研究 —— Corning SyntheGel基质胶系列
【新品】Tecan Revelo™ RNA-Seq High Sensitivity产品 优势 现货
探索SARS-CoV-2的奥秘:Revelo™ RNA-Seq High Sensitivity技术
Countstar Countstar Mira BF在类器官培养中利用
华雅思创生物代理Countstar:专业细胞成像分析设备的领先者
为什么选择康宁G418:细胞培养筛选的黄金标准
探索G418:细胞培养中的重要筛选工具