BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Kit
产品英文名称：BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Kit
BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Kit 英文简介
The BD™ Human and Mouse Pluripotent Stem Cell Analysis Kit provides a comprehensive research system for the reliable, in-depth characterization of cellular pluripotency and
differentiation state in heterogeneous stem cell mixtures. The total solution kit integrates pre-conjugated antibodies to markers for pluripotency and differentiation (including the
intracellular marker Oct 3/4), compensation beads, staining and fixation buffers, verified protocols, and software analysis guidelines.
BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Kit 的物质组成
SSEA-4 Alexa Fluor® 647, Oct3/4 PerCP-Cy™5.5, SSEA-1 PE; isotype control antibodies; BD Cytofix™ fixation buffer; BD Perm/Wash™ buffer; BD™ CompBead Plus compensation particles.
BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Kit 的保存条件
The entire BD Stemflow Human and Mouse Pluripotent Stem Cell Analysis Kit must be stored in the dark at 2° to 8°C. Do not freeze.
1.Provides in-depth, multimarker data (including expression of Oct 3/4) to characterize the pluripotency and differentiation state of stem cell subpopulations.
2.Delivers a streamlined solution for consistent experiments with pre-titrated fluorochrome conjugated antibodies, compensation beads, buffers, protocols, and
3.Facilitates compensation and scatter setup and conserves cells with BD CompBead Plus.
4.Provides flexibility to drop in additional fluorescently conjugated antibodies and to use cell lines expressing green fluorescent protein (GFP).
1.The BD Cytofix fixation buffer in this kit contains 4% paraformaldehyde (PFA) and is classified as harmful.The following risk and safety precautions should be
followed when handling this product:
2.Limited evidence of a carcinogenic effect (R40). May cause sensitization by skin contact (R43). Keep out of the reach of children (S2). Keep away from food,drink and animal feedingstuffs (S13). Wear suitabl eprotective clothing (S36). Wear suitable gloves (S37).If swallowed, seek medical attention immediately
and show this container or label (S46). Not recommended for interior use on large surface areas(S52).
3.The reagents in this kit contain sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water
before discarding to avoid accumulation of potentially explosive deposits inplumbing.
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