Human Neural Cell Sorting Kit
产品英文名称：Human Neural Cell Sorting Kit
BD Stemflow™ Human Neural Cell Sorting Kit 英文简介
The BD Stemflow™ Neural Cell Isolation Kit was designed to allow the isolation of neural stem cells (NSCs) derived from human pluripotent stem cells or the isolation of neurons and glial cells from differentiated NSCs. Following a variety of established neural induction protocols, NSCs are sorted from the heterogeneous cell culture utilizing the included antibody conjugates to five cell surface markers. This method greatly reduces the amount of variability between NSC isolations as opposed to traditional methods like manual isolation. In addition, four of the five included conjugates can be used to isolate neurons from glia from differentiated NSCs.
BD Stemflow™ Human Neural Cell Sorting Kit 的物质组成
CD15 PE-Cy™7, CD24 PE, CD44 PerCP-Cy™5.5, CD184 APC and CD271 PerCP-Cy5.5 antibodies; isotype controls; BD™ CompBead Plus compensation particles.
BD Stemflow™ Human and Mouse Pluripotent Stem Cell Analysis Kit的保存条件
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
1.Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
2.PerCP-Cy5.5–labelled antibodies can be used with FITC- and R-PE–labelled reagents in single-laser flow cytometers with no significant spectral overlap of PerCP-Cy5.5, FITC, and R-PE fluorescence.
3.PerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which may directly excite both PerCP and Cy5.5™. Werecommend the use of cross-beam compensation during data acquisition or software compensation during data analysis.
4.This product is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. If you require a commercial license to use this product and do not have one return this material, unopened to BD Biosciences, 10975 Torreyana Rd, San Diego, CA 92121 and any money paid for the material will be refunded.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
7. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
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