X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Medium
产品英文名称：X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Medium
产品中文名称：X-VIVO™ 10 化学成分确定的无血清造血干细胞培养基
X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Medium 研究背景
Lonza’s R&D efforts and collaboration with many clinical trials have provided Lonza with the tools and expertise to support the developments in adoptive immunotherapy, cancer therapy, genetic therapy and other cellular therapies. Lonza’s philosophy for the development of serum-free formulations for use in cellular therapy is to provide a nutritionally complete and balanced environment for the cells. We have not included any exogenous growth factors, artificial stimulators of cellular proliferation or undefined supplements.
The products are devoid of any protein-kinase C stimulators and are suitable for the investigation of second messenger systems in the activation of human and murine lymphocytes. The formulations are complete and contain pharmaceutical grade human albumin, recombinant human insulin and pasteurized human transferrin. All X-VIVO™ Medium products are manufactured under current GMPs and are listed with the FDA in a product Master File. Permission to cross-reference the Master File may be obtained by contacting the Product Manager.
X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Medium 简介
The X-VIVO™ 10 Medium formulation is designed to support the generation of Lymphokine Activated Killer (LAK) cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor Peripheral Blood Lymphocytes (PBL) at 1.0-3.0 x 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peripheral blood lymphocytes are incubated for three to ten days at a density of 1.0-6.0 x106 cells/ml in the presence of 100 - 1000 Cetus units of rIL-2. X-VIVO™ 10 Medium is available as a 1X liquid in several convenient configurations.
X-VIVO™ 10 化学成分确定的无血清造血细胞培养基特点
X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic Cell Medium 使用注意事项
1.The X-VIVO™ 10 formulation was designed to support the generation of Lymphokine Activated Killer (LAK) cells in a serum-free environment.
2.Please see Supplemental Information for additional hematopoietic cell applications.
3. X-VIVO™ 10, catalog number 04-743Q contains L-glutamine but does not contain gentamicin or phenol red.
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