Insulin ELISA kit
产品英文名称：Insulin ELISA kit
产品中文名称：胰岛素 ELISA 试剂盒
Insulin ELISA kit 的应用
本试剂盒是用于测定牛，人类和猪源的胰岛素。该试剂盒是用于研究和制造，未被批准用于人类或动物的诊断应用。对于需要更低的检测限的应用，Cygnus科技有限公司生产的超灵敏胰岛素ELISA试剂盒，货号# F280。那盒具有标准的25-1250pg／ml与限制的< 25pg／ml的范围。这种试剂盒可用于空腹的人血清中胰岛素水平的定量检测。
Insulin ELISA kit 的英文描述
The manufacture of products by various biotechnological processes such as cell or tissue culture can result in residual contamination of the desired product by components used in the culture media. The use of so called serum free defined media greatly reduces the number of potential contaminants but it may still be necessary to determine trace contamination levels of the proteins and growth factors used in these media. Most commercial formulations of serum free media contain significant amounts of albumin and transferrin either of bovine or human origin, and insulin from various species. When the intended product may be used as a therapeutic agent in humans or animals the product should be highly purified to avoid potential health risks or other problems that might result from trace contaminants. Efforts to reduce trace media contamination to the lowest levels practical require a highly sensitive and reliable analytical method. The Insulin assay is designed to provide a simple to use, precise, and highly sensitive method to detect Insulin contamination to less than 250 pg/mL. As such this kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
胰岛素 ELISA 试剂盒的使用步骤规则
This insulin assay is a two-site immunoenzymetric assay. Samples containing insulin are reacted simultaneously in microtiter strips coated with an affinity purified capture antibody. A second anti-Insulin antibody labeled alkaline phosphatase is reacted forming a sandwich complex of solid phase antibody- Insulin-Alkaline phosphatase labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with p-nitrophenyl phosphate (PNPP) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Insulin present. Accurate quantitation is achieved by comparing the signal of unknowns to Insulin standards assayed at the same time.
Insulin ELISA kit 的保存和稳定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
* Reconstituted wash solution is stable until the expiration date of the kit.
胰岛素 ELISA 试剂盒的使用注意事项
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