Protein A ELISA ki
产品英文名称：Protein A ELISA kit
产品中文名称：蛋白质 A ELISA 试剂盒
Protein A ELISA kit 的应用
Protein A ELISA kit 的英文描述
This ELISA incorporates some improvements over the earlier kits and is designed to better detect the newer, unnatural constructs of Protein A such as the MabSelect SuReTM ligand sold by GE Healthcare. If you are using a recombinant form of Protein A with very significant structural differences from natural Protein A such as MabSelect SuReTM you should use the Catalog #F400 kit. If you are evaluating our Protein A assays for the first time, we recommend using this kit, since it offers other robustness and procedural advantages over the older kits. The LOD is ~50pg/mL
We have improved the sample treatment procedure which reduces tedious pipetting while increasing throughput of the sample processing step. Validation of the new method shows excellent precision with improved overall robustness of the assay. This new method has demonstrated superior spike and recovery in problematic sample matrices and also overcomes 'front-to-back' drift reported by some labs. Labs equipped with microplate adaptable centrifuges can now process up to 96 tests simultaneously. Instead of using individual microfuge tubes, the new method uses a 96 well "PCR" Sample Treatment Plate and an adhesive seal, included in the kit. This procedure also minimizes problems of inadvertent disruption of the pelletized denatured proteins when handling microfuge tubes.
The Protein A assay is a two-site immunoenzymetric assay. Samples containing Protein A are first diluted with a sample denaturing buffer. This reagent dissociates the Protein A from the product antibody. Samples are then heated in a dry heating block or boiling water bath to denature and precipitate the product antibodies. After a centrifugation step to pellet the denatured product antibody, the samples are then reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second anti-Protein A antibody labeled directly with Horse Radish Peroxidase (HRP) enzyme is simultaneously reacted forming a sandwich complex of solid phase antibody-Protein A-HRP labeled antibody. After a wash step to remove any unbound reactants, the strips are then reacted with tetramethyl benzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of Protein A present. Accurate quantitation is achieved by comparing the signal of unknowns to Protein A standards assayed at the same tim.
Protein A ELISA kit 的保存和稳定性
* All reagents should be stored at 2C to 8C for stability until the expiration date printed.
* Reconstituted wash solution is stable until the expiration date of the kit.
Protein A ELISA 试剂盒的使用注意事项
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