BSA ELISA kit
产品英文名称：BSA ELISA kit
产品中文名称：BSA 牛血清白蛋白 ELISA 试剂盒
BSA ELISA kit 的应用
BSA 细胞 ELISA 试剂盒的英文描述
The manufacture of products by various biotechnological processes such as cell or tissue culture can result in residual contamination of the desired product by components used in the culture media. The use of so called serum free defined media greatly reduces the number of potential contaminants but it may still be necessary to determine trace contamination levels of the proteins and growth factors used in these media. Most commercial formulations of serum free media contain significant amounts of albumin and transferrin either of bovine or human origin, and insulin from various species. When the intended product may be used as a therapeutic agent in humans or animals the product should be highly purified to avoid potential health risks or other problems that might result from trace contaminants. Efforts to reduce trace media contamination to the lowest levels practical through optimal process design, validation, and final product testing require a highly sensitive and reliable analytical method. This BSA ELISA assay is designed to provide a simple to use, precise, and highly sensitive method to detect BSA contamination to less than 250 pg/mL. As such, this kit can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
BSA ELISA kit 的使用步骤规则
This BSA assay is a two-site immunoenzymetric assay. Samples containing BSA are reacted in microtiter strips coated with an affinity purified capture antibody. A second anti-BSA antibody labeled with the enzyme horseradish peroxidase (HRP) is reacted simultaneously forming a sandwich complex of solid phase antibody-BSA-HRP labeled antibody. After a wash step to remove any unbound reactants the strips are then reacted with Tetramethyl benzidine (TMB) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of BSA present. Accurate quantitation is achieved by comparing the signal of unknowns to BSA standards assayed at the same time.
BSA ELISA kit 的保存和稳定性
* All reagents should be stored at 2C to 8C for stability until the expiration date printed.
*The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
*Reconstituted wash solution is stable until the expiration date of the kit.
BSA ELISA kit 的使用注意事项
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