Mouse Total IgG ELISA kit
产品英文名称：Mouse Total IgG ELISA kit
产品中文名称：小鼠总 IgG 抗体 ELISA 试剂盒
Mouse Total IgG ELISA kit 的应用
Mouse Total IgG ELISA kit 的英文描述
The antibodies used in this kit are mouse immunoglobulin heavy and light chain specific. They do not cross react significantly with immunoglobulins from other species but they will cross react to varying degrees with other mouse immunoglobulins such as all IgG isotypes, IgA, and IgM. All of the Mouse Immunoglobulin ELISA kits can also be used to quantitate mouse immunoglobulin levels in serum of other animals administered mouse monoclonal antibodies in research studies.
Monoclonal antibodies are used in many applications such asdiagnostic or therapeutic agents as well as affinity supports for immunoaffinity purification of various products. These applications frequently require a sensitive assay to accurately quantitate the concentration of these antibodies in complex sample matrices. The antibodies used in this kit are mouse immunoglobulin heavy and light chain specific. They do not cross react significantly with immunoglobulin from other species but they will cross react to varying degrees with other mouse immunoglobulins such as all IgG isotypes, IgA, and IgM. The presence of high concentrations of mouse IgM and IgA immunoglobulin types in the sample can interfere in the ability of
this kit to quantitate mouse IgG. Cygnus Technologies provides kits for IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM immunoglobulins. See ordering information on the last page.
小鼠总 IgG 抗体 ELISA 试剂盒的使用步骤规则
The Mouse IgG assay is a two-site immunoenzymetric assay. Samples containing mouse IgG are reacted in microtiter strips coated with an affinity purified capture antibody. A second alkaline phosphatase enzyme labeled goat anti-mouse immmunoglobulin antibody is reacted, forming a sandwich
complex of solid phase antibody-mouse immunoglobulinenzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate p-nitrophenyl phosphate (PNPP) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly
proportional to the concentration of mouse IgG present. Accurate quantitation is achieved by comparing the signal of unknowns to IgG standards assayed at the same time.
Mouse Total IgG ELISA kit 的保存和稳定性
1 All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
2 The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
3 Reconstituted wash solution is stable until the expiration date of the kit.
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