Mouse IFN-γ ELISA Kit II
产品英文名称：Mouse IFN-γ ELISA Kit II
产品中文名称：小鼠干扰素-γ ELISA 试剂盒 II
Mouse IFN-γ ELISA Kit II 产品介绍
Interferon-γ( IFN-γ ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors.1,2 In addition to its antiviral effects, IFN--γcan upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN--γexerts strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve IFN--γ’s capacity to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. The BD Biosciences Pharmingen recombinant mouse IFN--γpresents as a 30 kD homodimeric protein, as shown by SDS-PAGE analysis.
Mouse IFN-γ ELISA Kit II 检测规则
The BD OptEIA test is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for mouse IFN- coated on a 96-well plate. Standards and samples are added to the wells, and any IFN-present binds to the immobilized antibody. The wells are washed and a mixture of biotinylated anti-mouse IFN-antibody and streptavidin-horseradish peroxidase is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is added, which produces a blue color at levels in direct proportion to the amount of IFN-present in the initial sample. The Stop Solution changes the color from blue to yellow, and the wells are read at 450 nm.
Mouse IFN-γ ELISA Kit II 保存方法
1. Store unopened kit at 2 – 8°C. Do not use kit after expiration date.
2. Before use, bring all reagents to room temperature (18 – 25°C).
Immediately after use, return to proper storage conditions.
3. Lyophilized standards are stable until kit expiration date. After reconstitution,
use freshly reconstituted standard within 12 hours (stored at 2 – 8°C).
Mouse IFN-γ ELISA Kit II 的优越性
•适用于冷冻/解冻的生物样品 •与其他检验方法兼容。例如，细胞分析。BD™ ELISPOT可以转移克隆，增殖实验，流式细胞术或其他分析方法。
Mouse IFN-γ ELISA Kit II 使用注意事项
1. Reagents that contain preservatives may be toxic if ingested, inhaled, or come in contact with the skin.
2. Avoid contact of skin, eyes, or clothing with Stop Solution or Substrate Reagents.
3. Handle all serum and plasma specimens in accordance with NCCLS guidelines for preventing transmission of blood-borne infections.
4. TMB One-Step Substrate Reagent is toxic if inhaled or swallowed. Avoid contact with skin. Keep container tightly closed. In case of an accident or if you feel unwell, seek medical advice immediately.
5. Standard/Sample Diluent and ELISA Diluent contain 0.09% sodium azide. Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid
accumulation of potentially explosive deposits in plumbing.
6. The Wash Concentrate contains 16 % Sodium Chloride and is an irritant.
7. The Stop Solution contains 11.5% Phosphoric Acid and is a corrosive solution.
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