EasySep™ Human Plasmacytoid DC Enrichment kit
产品英文名称: EasySep™ Human Plasmacytoid DC Enrichment kit
产品中文名称: EasySep™ 人类浆细胞DC富集试剂盒
EasySep™ Human Plasmacytoid DC Enrichment kit 产品英文描述
The EasySep™ Human Plasmacytoid DC Enrichment Kit is designed to isolate plasmacytoid dendritic cells (pDC) from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-pDCs and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
• EasySep™ Human Plasmacytoid DC Enrichment Cocktail, 2.0 mL
• EasySep™ Magnetic Particles, 8 x 1.0 mL
• Anti-Human CD32 (FcγRII) Blocker, 1.6 mL
EASYSEP™ HUMAN PLASMACYTOID DC ENRICHMENT COCKTAIL
EASYSEP™ D MAGNETIC PARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
ANTI-HUMAN CD32 (FcγRII) BLOCKER
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been
sterility tested. No preservative has been added. Do not freeze this product.
EasySep™ Human Plasmacytoid DC Enrichment kit 实验资料
Typical EasySep® Human pDC Enrichment Profile
Starting with 0.2 - 0.9% pDC in PBMC, the pDC content of the enriched fraction typically ranges from 87 - 97% purity based on the pDC phenotype of Lineage (CD3, CD14, CD16, CD19, CD20, CD34, CD56) negative, HLA-DR positive, and CD304 (BDCA-4) positive.
Typical EasySep™ Procedure for Human Cells
Stemcell 07801 Lymphoprep™ Density Gradient Medium for the Isolation of Mononuclear Cells
Stemcell 18000 EasySep™ Magnet Immunomagnetic Column-Free Magnet
Stemcell 18001 The Big Easy EasySep™ Magnet Immunomagnetic Column-Free Magnet
Stemcell 20000 RoboSep™ RoboSep™ - The Fully Automated Cell Separator
Stemcell 60012 Anti-Human CD32 Antibody, Clone IV.3 Mouse Monoclonal Antibody Against Human CD32
Stemcell 60018 Anti-Human CD45 Antibody, Clone HI30 Mouse Monoclonal Antibody Against Human, Chimpanzee CD45
Stemcell 60026 Anti-Dextran Antibody, Clone DX1 Mouse Monoclonal Antibody Against Dextran
For research use only. Not intended for human or animal diagnostic or therapeutic uses.
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