RoboSep™ Human Eosinophil Enrichment Kit with Filter Tips
产品英文名称: RoboSep™ Human Eosinophil Enrichment Kit with Filter Tips
产品中文名称: RoboSep™ 带有过滤嘴的人嗜酸性粒细胞富集试剂盒
RoboSep™ Human Eosinophil Enrichment Kit with Filter Tips 产品英文描述
The EasySep™ Human Eosinophil Enrichment Kit is designed to isolate eosinophils from a polymophonuclear cell-rich fraction of peripheral blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD19, CD20, CD36, CD56, CD123, glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. The enrichment kit is compatible with cells prepared using either Lymphoprep™ (Catalog 07801) centrifugation (preferred for higher cell purities) or HetaSep™ (Catalog #07906) sedimentation (preferred for faster, lysis-free sample processing).
The isolation of eosinophils from a polymorphonuclear cell-rich fraction by negative selection
RoboSep™ Human Eosinophil Enrichment Kit with Filter Tips 产品组份
• EasySep™ Human Eosinophil Enrichment Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 3 x 1.0 mL
EasySep® Negative Selection Human Eosinophil Enrichment Cocktail
Stable at 4°C for 2 years. Do not freeze this product. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep® Magnetic Nanoparticles
Stable at 4°C for 2 years. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
RoboSep™ Human Eosinophil Enrichment Kit with Filter Tips 实验资料
FACS Profile Results Using EasySep® Human Eosinophil Enrichment Kit
Starting with freshly prepared PMNCs, the eosinophil content of the enriched fraction typically ranges from 90% - 99% (gated on CD45).
Typical EasySep™ Procedure for Human Cells
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