MesenCult™-SF Culture Kit
产品英文名称：MesenCult™-SF Culture Kit
MesenCult™-SF Culture Kit 产品英文描述
MesenCult™-SF Culture Kit is a standardized, serum-free system for the culture of human mesenchymal stem cells (MSCs). It consists of MesenCult™-XF Medium (Catalog #05420) and MesenCult™-SF Attachment Substrate (Catalog #05424) and is optimized for the expansion of MSCs in vitro as well as their enumeration using the colony-forming unit - fibroblast (CFU-F) assay. This product also supports long-term growth of MSCs and cells maintain multi-lineage differentiation potential. The MesenCult™-SF Culture Kit must be used in conjunction with the MesenCult™-ACF Dissociation Kit (Catalog #05426). Components of the MesenCult™-ACF Dissociation Kit are prescreened and tested for optimal cell adherence when cells are passaged and cultured with MesenCult™-XF Medium. MesenCult™-XF Medium must be supplemented with L-glutamine.
• Mesenchymal stem cell expansion from primary human bone marrow
• Long-term culture of mesenchymal stem cells (greater than 8 passages)
• Detection and enumeration of CFU-F from primary human bone marrow and culture-expanded mesenchymal stem cells
MesenCult™-SF Culture Kit 产品组分
• MesenCult™-XF Basal Medium, 400 mL (Catalog #05421)
• MesenCult™-XF Supplement (5X), 100 mL (Catalog #05422)
• MesenCult™-SF Attachment Substrate, 5 mg (Catalog #05424)
MesenCult™-SF Culture Kit 保存方法
05421 MesenCult™-XF Basal Medium ,400 mL
Product stable at 2 - 8°C for one year from date of manufacture as indicated on label.
05422 MesenCult™-XF Supplement (5X) ,100 mL
Product stable at -20°C for one year from date of manufacture as indicated on label. Do not store at 2 - 8°C.
Human bone marrow-derived MSCs cultured in MesenCult®-XF expand faster than cells cultured in a traditional serum-based medium (n = 3; Mean ± SEM)
Human bone marrow-derived MSCs cultured in MesenCult®-XF display multi-lineage differentiation potential
A. Oil red O staining of adipocytes generated from passage 1 MSCs previously cultured in MesenCult®-XF.B. Alizarin Red detection of Ca2+ deposits indicates the formation of bone structures in cells generated from passage 4 MSCs previously cultured in MesenCult®-XF.C. Alcian Blue staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult®-XF.D. Collagen II staining of chondrocytes generated from passage 2 MSCs previously cultured in MesenCult®-XF.
Phenotype of culture-expanded human MSCs cultured in MesenCult®-XF
Stemcell 05420 MesenCult™-XF Medium Defined, Xeno-Free Medium for Human Mesenchymal Stem Cells
Stemcell 05424 MesenCult™-SF Attachment Substrate Serum-Free Attachment Substrate for Human Mesenchymal Stem Cells
Stemcell 05426 MesenCult™-ACF Dissociation Kit Dissociation Kit for Human Mesenchymal Stem Cells
Stemcell 05411 MesenCult™ Proliferation Kit (Human) Medium for Detection of CFU-F and Expansion of Human Mesenchymal Stem Cells
Stemcell 18659 EasySep™ Human CD271 Selection Kit Immunomagnetic Positive Selection Kit
Stemcell 07100 L-Glutamine L-Glutamine (200 mM)
For research use only. not intended for human or animal diagnostic or therapeutic uses.
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