RoboSep™ Human CD45 Depletion Kit with Filter Tips
产品英文名称：RoboSep™ Human CD45 Depletion Kit with Filter Tips
RoboSep™ Human CD45 Depletion Kit with Filter Tips 产品英文描述
The EasySep™ Human CD45 Depletion Kit is designed to deplete CD45+ cells from ammonium chloride lysed blood or bone marrow, or from fresh or previously frozen human peripheral blood mononuclear cells. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD45 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD45 antigen is expressed on all hematopoietic cells except erythrocytes and platelets.
• EasySep™ Human CD45 Depletion Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 2 x 1.0 mL
RoboSep™ Human CD45 Depletion Kit with Filter Tips 推荐使用
The depletion of human CD45+ cells from fresh or previously frozen human peripheral blood mononuclear cells
EasySep™ Human CD45 Depletion Cocktail
Stable at 4°C for 2 years. Do not freeze this product. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep™ Magnetic Particles
Stable at 4°C for 2 years. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
FACS Profile Results With EasySep® Human CD45 Cell Depletion Kit
Starting with whole blood, the depletion of CD45+ cells is typically 4.0 log. Plots were gated on Glycophorin A negative cells to exclude residual red blood cells. Ber-EP4 is an antibody against an epithelial cell surface antigen.
Typical EasySep™ Procedure for Human Cells
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