产品英文名称：CD4+CD25+ Regulatory T Cell Isolation Kit, mouse   
产品中文名称：美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒

美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒产品英文描述：
The CD4+CD25+ Regulatory T Cell Isolation Kit was developed for the isolation of CD4+CD25+ regulatory mouse T cells from single-cell suspensions of spleen and lymph nodes. The isolation is performed in a two-step procedure.
CD4+CD25+ immunoregulatory T cells have been shown to actively suppress immune responses against autologous and foreign antigens in vivo and in vitro. CD25, the IL-2Rα chain, is also expressed on activated CD4+ T cells, CD8+ T cells, dendritic cells, and B cells.

Miltenyi 美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒研究背景：
The CD4+CD25+ Regulatory T Cell Isolation Kit was developed for the isolation of mouse CD4+CD25+ regulatory T cells from singlecell suspensions of spleen or lymph nodes.
CD4+CD25+ immunoregulatory T cells have been shown to actively suppress immune responses against autologous and foreign antigens in vivo and in vitro. CD25, the IL-2Rα chain, is also expressed on activated CD8+ T cells, dendritic cells (DCs), and B cells.
The kit contains a cocktail of lineage specific biotin-conjugated antibodies against CD8 (Ly-2), CD11b (Mac-1), CD45R (B220),CD49b (DX5), Ter-119, and Anti-Biotin MicroBeads for depletion of non-CD4+ T cells, as well as CD25-PE and Anti-PE MicroBeads for subsequent positive selection of CD4+CD25+ regulatory T cells.

美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒产品介绍：
Components
1 mL CD4+CD25+ Regulatory T Cell Biotin-Antibody Cocktail, mouse:
Cocktail of biotin-conjugated monoclonal
anti-mouse antibodies against:
CD8a (Ly-2; isotype: rat IgG2a),
CD11b (Mac-1; isotype: rat IgG2b),
CD45R (B220; isotype: rat IgG2a),
CD49b (DX5; isotype: rat IgM)
and Ter-119 (isotype: rat IgG2b).
2 mL Anti-Biotin MicroBeads:
MicroBeads conjugated to monoclonal antibiotin antibody (isotype: mouse IgG1)
1 mL CD25-PE, mouse:
Monoclonal anti-mouse CD25 antibody conjugated to R-Phycoerythrin (PE) (clone: 7D4;isotype: rat IgM)
1 mL Anti-PE MicroBeads:
MicroBeads conjugated to monoclonal anti-PE antibodies (isotype: mouse IgG1)
Size
For 109 leukocytes, up to 100 separations.
Product format All components are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage
Store protected from light at 2–8 °C. Do not freeze.
The expiration date is indicated on the vial label.

Miltenyi 美天旎小鼠CD4+ CD25+调节性T细胞分离试剂盒产品应用：
Isolation of CD4+CD25+ regulatory T cells from single-cell suspensions of spleen or lymph nodes for:
• co-culture experiments with DCs to study priming of DCs for tolerance induction in vitro and after adoptive transfer of primed DCs in vivo.
• adoptive transfer experiments (e.g. from UV-exposed mice) to analyze the role of regulatory T cells during induction and elicitation of hapten-specific tolerance.