Human IGF-I Quantikine ELISA Kit
产品英文名称：R&D DG100 Human IGF-I Quantikine ELISA Kit
产品中文名称：R&D DG100 人胰岛素样生长因子-I 定量酶联免疫试剂盒
R&D DG100 Human IGF-I Quantikine ELISA Kit 产品简介
R&D DG100 Human IGF-I Quantikine ELISA Kit 是一个3.5小时的固相酶联免疫测定法是设计来检测吸附在细胞培养物上清，血清和血浆中的人体IGF-I的。它包含大肠杆菌表达的重组人IGF-Ⅰ，并已被证明能准确地定量重组因子。结果显示，使用自然的人类IGF-I 获得的平行曲线和使用Quantikine试剂盒标准得到的标准曲线呈线性。这些结果表明，本试剂盒可以被用来确定自然表达IGF-I的相对质量值。
R&D DG100 人胰岛素样生长因子-I 定量酶联免疫试剂盒产品参数
Assay Type:Solid Phase Sandwich ELISA
Format:96-well strip plate
Assay Length:3.5 hours
Sample Type & Volume Required Per
Well:Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)
Assay Range:0.094 - 6 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
Specificity:Natural and recombinant human IGF-I
Cross-reactivity:< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference:No significant interference observed with available related molecules.
R&D DG100 Human IGF-I Quantikine ELISA Kit 保存方法
Store the unopened kit at 2-8 °C. Do not use past kit expiration date.Store in a cool, dry place. Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.
R&D DG100 人胰岛素样生长因子-I 定量酶联免疫试剂盒实验操作程序
1.Prepare all reagents, standard dilutions, and samples as directed in the product insert.
2.Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
150 µL Assay Diluent
3.Add 150 µL of Assay Diluent to each well.
50 µL Standard, Control, or Sample
4.Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at 2-8 °C for 2 hours.
5.Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
200 µL cold Conjugate
6. Add 200 µL of cold Conjugate to each well. Cover with a new plate sealer, and incubate at 2-8 °C for 1 hour.
7.Aspirate and wash 4 times.
200 µL Substrate Solution
8. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
50 µL Stop Solution
9.Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
R&D DG100 Human IGF-I Quantikine ELISA Kit 产品英文描述
R&D DG100 Human IGF-I Quantikine ELISA Kit is a 7.6 kDa, 70 amino acid (aa) polypeptide with threeinternal disulfide bonds. The sequence of human IGF-I is identical to that of bovine and porcineIGF-I, and it is 70% identical to human IGF-II. IGF-I is a single-chain molecule with about50% identity to the sequences of the A- and B-chains of human insulin. For reviews of IGF-Isee references 1-3.
While IGF-I has very complex functions, it appears to largely mediate the actions of growthhormone. Thus, IGF-I is important in prenatal development, growth to adulthood, and metabolic control. It induces amino acid uptake, protein synthesis, and glucose utilization. It is an important mitogen and regulator of the cell cycle and apoptosis. IGF-I is produced primarily by hepatocytes, serving an endocrine function. It is also produced by many other cells, where it may act in an autocrine or paracrine manner. Serum levels of IGF-I have been reported to increase from birth to puberty, followed by a slow decline through adulthood (4).
There are two receptors for the IGFs; type I IGF receptor, which signals through a tyrosine kinase, and type II IGF receptor, which is identical to the mannose-6-phosphate receptor and may not signal. The action of IGF on its receptors is very complex, with control by at least six IGF-binding proteins (IGFBP-1 through IGFBP-6). IGFBP-3 binds over 90% of the total IGF in serum in a complex of IGF, IGFBP, and an acid-labile subunit. This ternary complex greatly stabilizes IGF in the circulation, changing the half-life from minutes to hours. In addition,IGFBP's modulate the action of IGF on the membrane receptors. Adding to the complexity is a family of proteases that act on IGFBPs, modifying their affinity for IGF or completely eliminating the IGFBPs. The interactions of IGF, IGFBP, IGFBP proteases, and IGF receptors are referred to as the IGF axis. The IGF axis affects many primary physiological and pathological processes,including development, growth, metabolic regulation, tumorigenesis, atherosclerosis, and angiogenesis.
The Quantikine Human IGF-I Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-I in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-I showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that R&D DG100 Human IGF-I Quantikine ELISA Kit can be used to determine relative mass values for naturally occurring IGF-I.
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
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