产品英文名称：Recombinant Human IL‑2 GMP
产品中文名称：重组人白细胞介素-2GMP
 

R&D 重组人白细胞介素-2GMP Recombinant Human IL‑2 GMP产品英文描述：
Source: E. coli-derived, Ala21-Thr153, with an N-terminal Met
Accession #: P60568
N-terminal Sequence Analysis: Met-Ala21-Pro-Thr-Ser-Ser-Ser-Thr-Lys-Lys
Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin Level: < 0.01 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass: 15.5 kDa (monomer)
Activity: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds.): IRL Press. 295.
The ED50 for this effect is typically 0.05-0.25 ng/mL.
Formulation: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.
Reconstitution: Reconstitute at 100 µg/mL in 100 mM Acetic Acid.
Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
·12 months, -20 to -70 °C as supplied.
·1 month, 2 to 8 °C under sterile conditions after reconstitution.
·3 months, -20 to -70 °C under sterile conditions after reconstitution.

R&D 重组人白细胞介素-2GMP Recombinant Human IL‑2 GMP产品相关数据：

1 μg/lane of GMP-grade Recombinant Human IL-2 (Catalog # 202-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver stain, showing a single band at 13 kDa.

GMP-grade Recombinant Human IL-2 (Catalog # 202-GMP) stimulates proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ is typically 0.05-0.25 ng/mL.

MALDI-TOF analysis of GMP-grade Recombinant Human IL-2 (Catalog # 202-GMP). The major peak corresponds to the calculated molecular mass, 15549 Da. The minor peak at 15764 Da is a matrix-associated artifact of the MALDI-TOF.

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