» WNT-5a,Mouse活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞TCF报告鉴定，纯度高
» 生物活性在共转Frizzled- 4 和 LRP- 5基因的HEK293 细胞鉴定
» WNT-5a,Mouse产品经过SDS-PAGE 和HPLC双层鉴定纯度大于85%
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of mouse WNT-5a is a monomeric protein containing 356 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa by SDS-PAGE analysis under non-reducing conditions. StemRD’s product is expressed from a mouse cell line, and purified with a proprietary process that is distinct from the published method.
• Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
• Biological Activity: The activity was determined by using a TCF reporter gene assay in 293 cells co-transfected with Frizzled-4 and LRP-5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (Milkels AJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.
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