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产品编号:HY-001342

TAKARA(宝日医)RetroNectin® 重组人纤粘连蛋白 T100B

RetroNectin® Recombinant Human Fibronectin Fragment

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  • 生产厂家: TAKARA厂家
  • 订购货号:
  • 保存温度: -20℃
  • 规格型号: 2.5mg/支
  • 产品编号: HY-001342
  • 产品种类: 特定细胞专用培养基

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产品英文名称:RetroNectin® Recombinant Human Fibronectin Fragment
产品中文名称:重组人纤粘连蛋白

 


RetroNectin® 重组人纤粘连蛋白 T100B产品简介:

RetroNectin的试剂已被广泛使用,以提高逆转录病毒和慢病毒基因转移方法进哺乳动物细胞中。
此外,RetroNectin的试剂增强的T淋巴细胞的增殖。 T细胞通常被扩大在白细胞介素-2(IL-2)的由刺激存在下与抗CD3抗体。在这种刺激步骤的加成RetroNectin的的显着地增加了T细胞的扩增效率。此外,T细胞扩大了与该方法包含较少分化的T细胞(幼稚T细胞)的比例高。幼稚T细胞具有分化成细胞毒性T细胞在体内的能力。


RetroNectin® 重组人纤粘连蛋白 T100B产品英文描述:
RetroNectin Reagent—Recombinant Human Fibronectin
Research using hematopoietic cells and other suspension cells has been limited in part by low efficiency of gene transfer (transduction and transfection) into these cell types. RetroNectin reagent promotes co-localization of lentivirus or retrovirus with target cells to dramatically enhance transduction efficiency. As a consequence, RetroNectin reagent is now the gold standard transduction enhancer for retroviral/lentiviral gene transfer to hematopoietic cells and holds great promise in clinical application, having been used in over 40 clinical trials.
RetroNectin reagent has been widely used to enhance retroviral and lentiviral gene transfer method into mammalian cells.
In addition, RetroNectin reagent enhances the proliferation of T lymphocytes. T cells are conventionally expanded in the presence of Interleukin-2 (IL-2) by stimulation with anti-CD3 antibody. The addition of RetroNectin in this stimulation step dramatically increases the efficiency of T cell expansion. In addition, T cells expanded with this method contain a high proportion of less-differentiated T cells (naive T cells). Naive T cells have the ability to differentiate into cytotoxic T cells in vivo.
Features
Enhanced gene transfer efficiency for retroviral or lentiviral transduction of suspension cells, hematopoietic stem cells, and other hard-to-infect cell types
Less toxicity than other commonly used transduction-enhancers, including polybrene and protamine
Fully recombinant peptide
Clinical grade RetroNectin (GMP) can be used for ex vivo clinical applications (investigational use only) - for GMP grade only please complete and return this form prior to purchase
Form :
Sterile solution containing 12.5 mM sodium citrate (pH6.2) and 1.25% sucrose
Source :
E. coli expressing human fibronectin fragment CH-296
Molecular weight :
62,617 Da (determined from amino acid sequence)
Storage : -20℃
Purity : > 90% by HPLC


RetroNectin® 重组人纤粘连蛋白 T100B相关产品订购信息:
Cat. #  Product                                              Size  
T100B  RetroNectin® Recombinant Human Fibronectin Fragment     2.5 mg  
T110A  RetroNectin® Precoated Dish                             10 Dishes  
T100A  RetroNectin® Recombinant Human Fibronectin Fragment     0.5 mg  
T201  RetroNectin® (GMP)                                     2.5 mg  

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