Human MIP-3β (CCL19) Pre-Coated ELISA Kit
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MIP-3β是一种CC趋化因子,在胸腺,淋巴结和活化的骨髓基质细胞中表达,通过CCR7受体传导。 MIP-3β是T和B淋巴细胞和骨髓祖细胞的趋化因子。
BioGems人MIP-3β(CCL19)预包被ELISA(酶联免疫吸附测定)试剂盒采用固相免疫法,专门设计用于检测MIP-3β(CCL19),96孔微板预包被MIP-3β特异鼠单克隆抗体(CCL19)。它含有大肠杆菌表达的重组MIP-3β(CCL19)。检测抗体是MIP-3β特异生物素化山羊多克隆抗体(CCL19)。该试剂盒是即用型试剂盒,已经过分析验证。
Human MIP-3β (CCL19) Pre-Coated ELISA Kit分析
 
 
Product Details
| Assay Type: | Solid Phase Sandwich ELISA: 96-Well Strip Plate | 
| Sensitivity: | <10pg/ml | 
| Range: | 15.6pg/ml-1000pg/ml | 
| Specificity: | Natural and recombinant Human MIP-3β (CCL19) | 
| Uniprot: | Q6IBD6 | 
| Interference: | No detectable cross-reactivity with other relevant proteins | 
| Storage Conditions: | Store the unopened product at 2 – 8 ℃. | 
Components组份
| Description | Quantity | Volume | 
| Anti-Human MIP-3β(CCL19) Pre-coated 96-well strip microplate | 1 | 12 strips of 8 wells | 
| Human MIP-3β (CCL19) Standard | 2 | 10 ng vial | 
| Human MIP-3β (CCL19) Biotinylated antibody (100x) | 1 | 130 µL | 
| Avidin-Biotin-Peroxidase Complex (100x) | 1 | 130 µL | 
| Sample Diluent | 1 | 30mL | 
| Antibody Diluent | 1 | 12mL | 
| Avidin-Biotin-Peroxidase Diluent | 1 | 12mL | 
| Wash Buffer Concentrate (Powder for 1000mL) | 1 | Pack | 
| Color Developing Reagent | 1 | 10mL | 
| Stop Solution | 1 | 10mL | 
| Plate Sealers | 4 | Each | 
Reproducibility 可重复性
| 
 | Intra-Assay | Inter-Assay | ||||
| Sample ID | 1 | 2 | 3 | 1 | 2 | 3 | 
| n= | 16 | 16 | 16 | 24 | 24 | 24 | 
| Mean Measured Concentration (pg/mL) | 97 | 293 | 583 | 117 | 374 | 627 | 
| Standard Deviation | 5.53 | 14.1 | 36.15 | 7.5 | 18.7 | 42.64 | 
| Consistency (%CV) | 5.7 | 4.8 | 6.2 | 6.4 | 5 | 6.8 | 
Catalog Number : BGK6IBD6
Description
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Human MIP-3β (CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β (CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β (CCL19). It contains E. coli-expressed recombinant MIP-3β (CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β (CCL19). The kit is analytically validated with ready to use reagents.
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