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产品编号:HY-001460

BioGems鼠MIP-3β(CCL19)预包被ELISA试剂盒,Murine MIP-3β (CCL19) Pre-Coated ELISA Kit

Murine MIP-3β (CCL19) Pre-Coated ELISA Kit

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  • 生产厂家: BioGems
  • 规格型号: 1 Kit
  • 产品编号: HY-001460
  • 产品种类: 细胞活力检测

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Catalog Number :BGK70460

MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.

The BioGems Murine MIP-3β (CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β (CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β (CCL19). It contains E. coli-expressed recombinant MIP-3β (CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β (CCL19). The kit is analytically validated with ready to use reagents.

 

MIP-3β是一种CC趋化因子,在胸腺,淋巴结和活化的骨髓基质细胞中表达,通过CCR7受体传导信号。MIP-3βTB淋巴细胞和骨髓祖细胞的趋化因子(chemoattractant

BioGemsMIP-3βCCL19)预包被ELISA(酶联免疫吸附测定)试剂盒采用固相免疫测定法,专门设计用于检测MIP-3βCCL19),其中96微孔板包被小鼠MIP-3β特异性单克隆抗体(CCL19)。 它含有大肠杆菌表达的重组MIP-3βCCL19)。 检测抗体是对MIP-3β特异的生物素化山羊多克隆抗体(CCL19)。该试剂盒经过分析验证,是即用型试剂。

 

 

Product Details

Assay Type:

Solid Phase Sandwich ELISA: 96-Well Strip Plate

Sensitivity:

<10pg/ml

Range:

15.6pg/ml-1000pg/ml

Specificity:

Natural and recombinant Murine MIP-3β(CCL19)

Uniprot:

O70460

Interference:

No detectable cross-reactivity with other relevant proteins

Storage Conditions:

Store the unopened product at  2 – 8 .

 

Components 组份

Description

Quantity

Volume

Anti-Murine MIP-3β (CCL19) Pre-coated 96-well strip microplate

1

12 strips of 8 wells

Murine MIP-3β (CCL19) Standard

2

10 ng vial

Murine MIP-3β (CCL19) Biotinylated antibody (100x)

1

130 µL

Avidin-Biotin-Peroxidase Complex (100x)

1

130 µL

Sample Diluent

1

30mL

Antibody Diluent

1

12mL

Avidin-Biotin-Peroxidase Diluent

1

12mL

Wash Buffer Concentrate (Powder for 1000mL)

1

Pack

Color Developing Reagent

1

10mL

Stop Solution

1

10mL

Plate Sealers

4

Each

 

Reproducibility 可重复性

 

Intra-Assay

Inter-Assay

Sample ID

1

2

3

1

2

3

n=

16

16

16

24

24

24

Mean Measured Concentration (pg/mL)

91

286

592

105

379

645

Standard Deviation

3.82

14.3

34.93

6.51

28.05

52.25

Consistency (%CV)

4.2

5

5.9

6.2

7.4

8.1

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