Murine MIP-3β (CCL19) Pre-Coated ELISA Kit
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Catalog Number :BGK70460
MIP-3β is a CC chemokine, expressed in the thymus, lymph nodes, and in activated bone marrow stromal cells that signals through the CCR7 receptor. MIP-3β is a chemoattractant for T and B lymphocytes, and myeloid progenitor cells.
The BioGems Murine MIP-3β (CCL19) Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure MIP-3β (CCL19) with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for MIP-3β (CCL19). It contains E. coli-expressed recombinant MIP-3β (CCL19). The detection antibody is a biotinylated goat polyclonal antibody specific for MIP-3β (CCL19). The kit is analytically validated with ready to use reagents.
MIP-3β是一种CC趋化因子,在胸腺,淋巴结和活化的骨髓基质细胞中表达,通过CCR7受体传导信号。MIP-3β是T和B淋巴细胞和骨髓祖细胞的趋化因子(chemoattractant)。
BioGems鼠MIP-3β(CCL19)预包被ELISA(酶联免疫吸附测定)试剂盒采用固相免疫测定法,专门设计用于检测MIP-3β(CCL19),其中96孔微孔板预包被小鼠MIP-3β特异性单克隆抗体(CCL19)。 它含有大肠杆菌表达的重组MIP-3β(CCL19)。 检测抗体是对MIP-3β特异的生物素化山羊多克隆抗体(CCL19)。该试剂盒经过分析验证,是即用型试剂。
Product Details
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Assay Type: |
Solid Phase Sandwich ELISA: 96-Well Strip Plate |
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Sensitivity: |
<10pg/ml |
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Range: |
15.6pg/ml-1000pg/ml |
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Specificity: |
Natural and recombinant Murine MIP-3β(CCL19) |
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Uniprot: |
O70460 |
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Interference: |
No detectable cross-reactivity with other relevant proteins |
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Storage Conditions: |
Store the unopened product at 2 – 8 ℃. |
Components 组份
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Description |
Quantity |
Volume |
|
Anti-Murine MIP-3β (CCL19) Pre-coated 96-well strip microplate |
1 |
12 strips of 8 wells |
|
Murine MIP-3β (CCL19) Standard |
2 |
10 ng vial |
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Murine MIP-3β (CCL19) Biotinylated antibody (100x) |
1 |
130 µL |
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Avidin-Biotin-Peroxidase Complex (100x) |
1 |
130 µL |
|
Sample Diluent |
1 |
30mL |
|
Antibody Diluent |
1 |
12mL |
|
Avidin-Biotin-Peroxidase Diluent |
1 |
12mL |
|
Wash Buffer Concentrate (Powder for 1000mL) |
1 |
Pack |
|
Color Developing Reagent |
1 |
10mL |
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Stop Solution |
1 |
10mL |
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Plate Sealers |
4 |
Each |
Reproducibility 可重复性
|
|
Intra-Assay |
Inter-Assay |
||||
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Sample ID |
1 |
2 |
3 |
1 |
2 |
3 |
|
n= |
16 |
16 |
16 |
24 |
24 |
24 |
|
Mean Measured Concentration (pg/mL) |
91 |
286 |
592 |
105 |
379 |
645 |
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Standard Deviation |
3.82 |
14.3 |
34.93 |
6.51 |
28.05 |
52.25 |
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Consistency (%CV) |
4.2 |
5 |
5.9 |
6.2 |
7.4 |
8.1 |
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