Protein A ELISA kit
产品英文名称：Protein A ELISA kit
产品中文名称：蛋白质 A ELISA 试剂盒
Protein A ELISA kit 的应用
本试剂盒是用于来自于自然（金黄色葡萄球菌）蛋白质配体和大肠杆菌重组表达构建结构的定量测定。该试剂盒只用于研究和制造，不用于人类或动物的诊断应用。 Cygnus制造两个其他蛋白质的检测试剂盒，货号F050H和F400。F050H优于F050产品抗体,当人类或其他显示干扰蛋白质检测的时候。我们的新的蛋白试剂盒F400将超过前两包的一些改进，旨在更好地发现新的蛋白质，如被GE卫生保健出售的不自然的MabSelect SuReTM配体。
Protein A ELISA kit 的英文描述
Protein A immobilized on various chromatography media is commonly used to purify antibodies. Even when covalently attached, Protein A can leach off of the chromatography support and co-elute with the antibody. For some applications such as the pharmaceutical use of the antibody, contamination with。Protein A must be minimized to avoid any adverse effects. There are several manufacturers of Protein A and Protein A chromatography supports. In addition to natural Protein A purified from S. aureus, there are also various recombinant constructs of Protein A typically expressed in E. coli. Some of these recombinant Protein A’s are essentially identical to natural Protein A. However, there are other unnatural recombinant constructs with very significant structural differences when compared to natural Protein A. GE Healthcare sells one such unnatural construct marketed as MabSelect SuRe TM. Because of the unique structure of MabSelect SuRe TM it is only about 20% reactive in our F050 and F050H kits. For this reason weoffer our newest Protein A kit, Cat # F400. That kit incorporates a new antibody as well as other changes allowing it to detect all forms of Protein A equally. Some product antibodies when complexed with the leached Protein A can interfere in the detection of Protein A when using the F050 kit. Like our F050H kit, Cat # F400 also utilizes a sample treatment step involving acid dissociation and heat denaturation to overcome any interference from product immunoglobulin. The Cygnus Technologies’ Protein A ELISA kits are designed to detect Protein A contamination to less than one part per million. As such, these kits can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.
蛋白质 A ELISA 试剂盒的使用步骤规则
The Protein A assay is a two-site immunoenzymetric assay. Samples containing Protein A are reacted in microtiter strips coated with a polyclonal anti-Protein A capture antibody. A second biotinylated Monoclonal anti-Protein A antibody is reacted forming a sandwich complex of solid phase antibody-Protein A-biotinylated antibody. After a wash step to remove any unbound reactants, the strips are then reacted with Streptavidin labeled with Alkaline Phosphatase enzyme which will bind to any of the biotinylated antibody bound to the strip. After another wash step to remove unbound Streptavidin: alkaline phosphatase, the plates are then reacted with pnitrophenyl phosphate (PNPP) substrate. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of Protein A present. Accurate quantitation is achieved by comparing the signal of unknowns to Protein A standards assayed at the same time.
Protein A ELISA kit 的保存和稳定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
* Reconstituted wash solution is stable until the expiration date of the kit.
Protein A ELISA 试剂盒的使用注意事项
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