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ProSpec Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重组人肿瘤坏死因子α

Recombinant Human Tumor Necrosis Factor-alpha

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  • 生产厂家: ProSpec
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  • 保存温度: -18℃
  • 规格型号: 10ug,50ug,1mg
  • 产品编号: HY-001116
  • 产品种类: 特定细胞专用培养基

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英文名称:Recombinant Human Tumor Necrosis Factor-alpha
中文简称:【TNF a 】重组人肿瘤坏死因子α

 

Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重组人肿瘤坏死因子α 细胞因子产品描述:

肿瘤坏死因子-α(Tumor Necrosis Factor-alpha,TNF-α)是一种主要由单核-吞噬细胞产生的单核因子,不仅具有选择性地杀伤某些肿瘤细胞,而且有多种免疫调节作用。生理条件下具有生物学活性的人肿瘤坏死因子α(hTNF-α)是一个紧密的三聚体,其活性位点位于两个相邻亚基之间的V型结构域,hTNF-α有两种不同的受体,分别介导不同的生物学活性,在对hTNF-α结构与功能研究的基础上,人们对其N末端、C末端和p75受体结合位点进行基因改造。研制了一系列衍生物,以提高hTNF-α的抗肿瘤活性,降低毒副反应。
人肿瘤坏死因子α重组蛋白是在大肠杆菌中重组表达的,是一条单链、非糖基化的多肽链,包含有158个氨基酸,分子量为17483.77Da。该人肿瘤坏死因子α重组蛋白最终由标准的色谱技术纯化出。
来源宿主---大肠杆菌中重组表达

 

Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重组人肿瘤坏死因子α 细胞因子产品使用须知:

溶解:提供的人肿瘤坏死因子α(TNF a)产品为过滤灭菌的白色冻干粉末;该冻干粉末中每毫克人肿瘤坏死因子是从20mM PBS(pH 7.2),10mM NaCl溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml,后继实验过程中可根据实验要求进一步稀释。
保存:人肿瘤坏死因子α(TNF a )在室温可保存3周,或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天;如果想延长保存时间,建议分装(加载体蛋白如0.1% HSA或 BSA)后置于-18℃冻存。请避免反复冻融。

 

Recombinant Human Tumor Necrosis Factor-alpha 【TNF a 】重组人肿瘤坏死因子α 细胞因子产品英文描述

Synonyms 

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.


Introduction
 

Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages.
TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells.
Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.


Description 

Tumor Necrosis Factor-a Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids (157 a.a. of the mature human TNF-alpha and an N-terminal methionine) and having a molecular mass of 17.5kDa.
The TNF-alpha is purified by standard chromatographic techniques.


Source  (来源)

Escherichia Coli.


Physical Appearance   (外观)

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation  

1mg of TNF-alpha Human contain 20mM PB, pH-7.2, and 100mM NaCl.


Solubility   (溶解度)

It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


Stability     (稳定性)

Lyophilized Tumor Necrosis Factor-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.


Purity       (纯度)

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.


Amino acid sequence    (氨基酸序列)

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA E AKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL.


Biological Activity     (生物活性)

The Specific Activity is >5.0×107 IU/mg as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D.

 

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