产品英文名称: EasySep™ RosetteSep™ Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells
产品中文名称: EasySep™ RosetteSep™ CD4+ CD127lowCD25+ T细胞完整试剂盒

EasySep™ RosetteSep™ Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells 产品英文描述
The Complete Kit for Human CD4+CD127lowCD25+ Regulatory T Cells is a simple two-step method that is designed to isolate CD4+CD127lowCD25+ cells from whole blood or buffy coat. CD4+CD127low T cells are enriched from whole blood or buffy coat by negative selection using RosetteSep™ CD4+CD127low T Cell Pre-Enrichment Cocktail. The RosetteSep™ cocktail contains a combination of antibodies that is directed against non-CD4+CD127low T cells and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Ficoll-Paque™ PLUS (Catalog #07957), the unwanted cells pellet along with the RBCs. The purified cells are present as an enriched population at the interface between the plasma and the buoyant density medium. The enriched cells are then selected for CD25+ cells by positive selection using EasySep™ Human CD25 Positive Selection Kit. Cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

EasySep™ RosetteSep™ Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells 推荐使用
The isolation of human CD4+CD127lowCD25+ regulatory T cells from whole blood or buffy coat by positive selection

EasySep™ RosetteSep™ Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells 产品组成
• RosetteSep™ Human CD4+CD127low T Cell Pre-Enrichment Cocktail, 5 x 2 mL
• EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
• EasySep™ Magnetic Particles, 1 mL

 EasySep™ RosetteSep™ Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells 保存方法
EASYSEP® HUMAN CD25 POSITIVE SELECTION COCKTAIL AND
EASYSEP® MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 – 25°C), and should be refrigerated upon receipt.

EasySep™ RosetteSep™ Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells 订购信息
Stemcell  15862  Complete Kit for Human CD4+CD25+ T Cells  Immunomagnetic Positive Selection Kit Stemcell  15022  RosetteSep™ Human CD4+ T Cell Enrichment Cocktail  Immunodensity Negative Selection Cocktail 
Stemcell  18231  EasySep™ Human CD25 Positive Selection Kit  Immunomagnetic Positive Selection Kit Stemcell  10512  CD25/IL-2R-alpha Antibody, Clone 2A3, PE-Conjugated  Mouse Monoclonal Antibody to Human CD25 (IL-R2α) - PE Conjugated 
Stemcell  07801  Lymphoprep™  Density Gradient Medium for the Isolation of Mononuclear Cells 
Stemcell  15705  RosetteSep™ DM-L Density Medium  Density Gradient Medium 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator    
For research use only. Not intended for human or animal diagnostic or therapeutic uses.