RoboSep™ Human Buffy Coat CD14 Positive Selection Kit with Filter Tips
产品英文名称: RoboSep™ Human Buffy Coat CD14 Positive Selection Kit with Filter Tips
产品中文名称: RoboSep™ 带有过滤嘴的人白膜CD14阳性选择试剂盒
The EasySep™ Human Buffy Coat CD14 Positive Selection Kit is designed to isolate CD14+ cells from freshly prepared buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD14 antigen is strongly expressed on monocytes and is weakly expressed on granulocytes. It is also expressed on most tissue macrophages.
RoboSep™ Human Buffy Coat CD14 Positive Selection Kit with Filter Tips Recommended for
The isolation of human CD14+ cells from buffy coat by positive selection
• EasySep™ Human Buffy Coat CD14 Positive Selection Cocktail, 3 x 1.0 mL
• EasySep™ Magnetic Particles, 3 x 1.0 mL
• EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL
RoboSep™ Human Buffy Coat CD14 Positive Selection Kit with Filter Tips 保存方法
EasySep® Human Buffy Coat CD14 Positive Selection Cocktail.
Stable at 4°C for 2 years. Do not freeze this product. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep® Whole Blood Magnetic Nanoparticles.
Stable at 4°C for 2 years. Contents sterile in unopened tube. This product may be shipped at room temperature, and should be refrigerated upon receipt.
EasySep® RBC Lysis Buffer 10X Concentrate
10X Concentrate is stable at room temperature for 2 years. Store at room temperature. 1X Lysis Buffer is stable at 4°C for 3 months. Store at 2 - 8°C. Do not freeze.
FACS Histogram Results Using EasySep® Human Buffy Coat CD14 Positive Selection Kit
Starting with buffy coat, the CD14+ cell content of the selected fraction typically ranges from 96.5% - 99.6%.*Red blood cells were removed by lysis prior to flow cytometry.
Typical EasySep™ Procedure for Human Cells
Stemcell 07800 Ammonium Chloride Solution Reagent for the Lysis of Red Blood Cells
Stemcell 20110 EasySep™ Red Blood Cell Lysis Buffer Red Blood Cell Lysis Buffer
Stemcell 18001 The Big Easy EasySep™ Magnet Immunomagnetic Column-Free Magnet Stemcell 10406 CD14 Antibody, Clone MoP9, FITC-Conjugated Mouse Monoclonal Antibody to Human CD14 - FITC Conjugated
Stemcell 09600 StemSpan™ SFEM Serum-Free Medium for Expansion of Hematopoietic Cells
Stemcell 09650 StemSpan™ SFEM
Stemcell 20000 RoboSep™ RoboSep™ - The Fully Automated Cell Separator
Stemcell 19251 EasySep™ Human Pan-DC Pre-Enrichment Kit Immunomagnetic Negative Selection Kit
Stemcell 18000 EasySep™ Magnet Immunomagnetic Column-Free Magnet
For research use only. Not intended for human or animal diagnostic or therapeutic uses.
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