NutriStem® hESC XF medium for human ES & iPS Cell culture, with HSA
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胚胎/IPS干细胞无血清培养基产品信息:
NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, with HSA
货号:05-100-1A/B,人类胚胎干细胞无血清培养基(含人血清白蛋白)
AF NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, without HSA
货号: 05-102-1A/B,人类胚胎干细胞无血清培养基(不含人血清白蛋白)
人类胚胎干细胞研究是当前最热门的科研课题之一,经由无限制的增殖及适当的诱导分化后,胚胎细胞干细胞可分化成人体中各种类型的细胞,如神经细胞、心肌细胞及软骨细胞,这些特点造就胚胎干细胞未来在人类医学健康应用上不可估量的前景。
传统干细胞培养过程中必需添加的异种动物(非人类)成分,如牛血清或猪血清,使之无法完全排除人畜共染病毒、朊病毒(Prion)及支原体污染细胞 的可能性,这是造成人类干细胞进入临床应用的主要障碍之一。经由使用成分明确、全合成的无血清干细胞培养基可有效避免人畜共染疾病的问题,同时全合成培养 基无批间差异,培养条件容易保持一致,实验的重复性大为提高。应用无血清干细胞培养基可为干细胞在心血管疾病、神经性退化及癌症的临床治疗方面打开了一扇 大门。
为了满足科研与生物医药企业的需求,BI公司与以色列国家科学研究院(Technion-Israel Institute of Technology)的Dr. Itskovitz共同合作开发了zui新一代、拥有自主知识产权的人类胚胎干细胞(hESC)和诱导胚胎干细胞(iPSC)专用无血清完全培养基—— NutriStem®。
Dr. Joseph Itskovitz己在Stem Cells, Stem Cell Dev, PNAS, Nature及Science等杂志发表近百篇文章,并有三项胚胎干细胞培养与分化诱导国际,同时也是1998年*篇分离人类胚胎干细胞论文的共同作者,现任职于以色列国家科学研究院Rambam Medical Center。美国国家卫生研究院干细胞库中批准用于科研的22株人类胚胎干细胞由其团队提供了8株。
Embryonic stem cell lines derived from human blastocysts. J. A. Thomson, J. Itskovitz-Eldor, et. al., Science. Vol. 282, no. 5391, 1145-1147. 1998.
NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, with HSA
货号:05-100-1A/B,人类胚胎干细胞无血清培养基(含人血清白蛋白)
开瓶即可使用的完全培养基,不含异源动物成分,添加了医疗级的人血清白蛋白(HSA:Human Serum Albumin),以适用于无滋养层培养法(Feeder-Free),如Matrigel™或conditioned medium培养。
AF NutriStem® hESC XF, Xeno-Free medium for human ES & iPS Cell culture, without HSA
货号: 05-102-1A/B,人类胚胎干细胞无血清培养基(不含人血清白蛋白)
开瓶即可使用的完全培养基,不含任何动物来源成分,适用于鼠胚胎纤维原细胞(Mouse Embryonic Fibroblasts-MEF)及人包皮纤维原细胞(Human Foreskin Fibroblasts-HFF)滋养层培养法。
NutriStem®已发表参考文献:
1. Human and mouse adipose-derived cells support feeder-independent induction of pluripotent stem cells. Sugii et al. PNAS. Vol. 107, No 8, 3558-3563, 2009.
2. Current technology for the derivation of pluripotent stem cell lines from human embryos. Hasegawa et al., Cell Stem Cell. Vol. 6, 521-531, 2010.
3. Progress and challenges in optimiztion of human pluripotent stem cell culture. Ge et al., Current Stem Cell research & Therapy. Vol. 5, 207-214, 2010.
4. Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA. Warren et al., Cell Stem Cell. Vol. 7, 1-13, 2010.
5. Fibrin microbeads (FMB) loaded with mesenchymal cells support their long term survival while sealed at room temperature. Gorodetsky et al. Tissue Engineering Part C: Methods. Online Ahead of Editing, March 2011.
6. Site-specific gene correction of a point mutation in human iPS cells derived from an adult patient with sickle cell disease. Zou, et al. Blood. August 2011.
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