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产品编号:HY-000666

Cygnus Technologies F640 Pichia pastoris 2nd Generation HCP ELISA 试剂盒

Pichia pastoris 2nd Generation HCP ELISA kit

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  • 生产厂家: Cygnus Technologies
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  • 保存温度: 2~-8℃
  • 规格型号: 1 Kit
  • 产品编号: HY-000666
  • 产品种类: DMSO细胞冻存液

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产品描述 说明书【下载】 用户评论【0】

产品英文名称:Pichia pastoris 2nd Generation HCP ELISA kit
产品中文名称:毕赤酵母第二代 HCP 试剂盒

Pichia pastoris 2nd Generation HCP ELISA kit 的应用
本试剂盒是用于确定在毕赤酵母中的重组表达的宿主细胞生产的产品的毕赤酵母蛋白污染的存在。Cygnus卖毕赤酵母HCP检测的两种ELISA试剂盒。原盒F140,有一个非常广泛的反应性的肽表位,此外,还有一个小群体的抗体识别的抗原表位的毕赤酵母的糖基化。在重组蛋白产品同时糖化,有交叉反应情况下,可以导致在真正的hcp或不定的hcp浓度由于样本稀释线性估计潜在的缺乏。用F640试剂盒用的抗体是与F140试剂盒相同的多克隆抗体是除了已经承受额外的亲和吸附去除抗体的糖基化表位。该试剂盒是用于研究和制造使用,不用于人类或动物的诊断应用。

毕赤酵母第二代 HCP 试剂盒的英文描述
Our original F140 kit, has a very broad reactivity to peptide epitopes and in addition, also has a small population of antibodies that recognize Pichia glycosylation epitopes. In cases where the recombinant protein product is also glycosylated, there is potential for some cross reactivity that can result in either an over-estimation of true HCP or indeterminate HCP concentrations, due to the lack of sample dilutional linearity.
The antibody used in this kit is the same polyclonal antibody that is used in the F140 kit, except that it has been subjected to an additional affinity adsorption step to remove antibodies to glycosylation epitopes and is much more specific than the F140, Pichia pastoris HCP ELISA kit.

Pichia pastoris 2nd Generation HCP ELISA kit 的使用步骤规则
The Pichia pastoris assay is a two-site immunoenzymetric assay. Samples containing Pichia pastoris HCPs are reacted in microtiter strips coated with an affinity purified capture antibody. A second horseradish peroxidase (HRP) enzyme labeled anti-Pichia pastoris antibody is reacted simultaneously resulting in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of Pichia pastoris HCPs present.

毕赤酵母第二代 HCP 试剂盒的保存和稳定性
1 All reagents should be stored at 2℃ to 8C℃for stability until the expiration date printed.
2 Reconstituted wash solution is stable until the expiration date of the kit.

Pichia pastoris 2nd Generation HCP ELISA kit的使用注意事项
1 只用于研究或制造。
2 停止反应试剂是0.5N 硫酸。避免接触眼睛,皮肤,和服装。在用本试剂盒的试剂浓度,其他没有被认为是有害的。
3 本试剂盒只能由合格的技术人员使用。
 

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